Back to Search
Start Over
Effect of Buffer Conditions and Organic Cosolvents on the Rate of Strain-Promoted Azide-Alkyne Cycloaddition.
- Source :
-
The Journal of organic chemistry [J Org Chem] 2016 Aug 05; Vol. 81 (15), pp. 6816-9. Date of Electronic Publication: 2016 Jul 14. - Publication Year :
- 2016
-
Abstract
- We investigate the effect of buffer identity, ionic strength, pH, and organic cosolvents on the rate of strain-promoted azide-alkyne cycloaddition with the widely used DIBAC cyclooctyne. The rate of reaction between DIBAC and a hydrophilic azide is highly tolerant to changes in buffer conditions but is impacted by organic cosolvents. Thus, bioconjugation reactions using DIBAC can be carried out in the buffer that is most compatible with the biomolecules being labeled, but the use of organic cosolvents should be carefully considered.
Details
- Language :
- English
- ISSN :
- 1520-6904
- Volume :
- 81
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- The Journal of organic chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 27387821
- Full Text :
- https://doi.org/10.1021/acs.joc.6b01112