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Frontline Science: Tryptophan restriction arrests B cell development and enhances microbial diversity in WT and prematurely aging Ercc1 -/Δ7 mice.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 2017 Apr; Vol. 101 (4), pp. 811-821. Date of Electronic Publication: 2016 Jul 14. - Publication Year :
- 2017
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Abstract
- With aging, tryptophan metabolism is affected. Tryptophan has a crucial role in the induction of immune tolerance and the maintenance of gut microbiota. We, therefore, studied the effect of dietary tryptophan restriction in young wild-type (WT) mice (118-wk life span) and in DNA-repair deficient, premature-aged ( Ercc1 <superscript>-/Δ7</superscript> ) mice (20-wk life span). First, we found that the effect of aging on the distribution of B and T cells in bone marrow (BM) and in the periphery of 16-wk-old Ercc1 <superscript>-/Δ7</superscript> mice was comparable to that in 18-mo-old WT mice. Dietary tryptophan restriction caused an arrest of B cell development in the BM, accompanied by diminished B cell frequencies in the periphery. In general, old Ercc1 <superscript>-/Δ7</superscript> mice showed similar responses to tryptophan restriction compared with young WT mice, indicative of age-independent effects. Dietary tryptophan restriction increased microbial diversity and made the gut microbiota composition of old Ercc1 <superscript>-/Δ7</superscript> mice more similar to that of young WT mice. The decreased abundances of Alistipes and Akkermansia spp. after dietary tryptophan restriction correlated significantly with decreased B cell precursor numbers. In conclusion, we report that dietary tryptophan restriction arrests B cell development and concomitantly changes gut microbiota composition. Our study suggests a beneficial interplay between dietary tryptophan, B cell development, and gut microbial composition on several aspects of age-induced changes.<br /> (© Society for Leukocyte Biology.)
- Subjects :
- Animals
Bacteria metabolism
Bone Marrow metabolism
Diet
Female
Gastrointestinal Tract microbiology
Immunologic Memory
Lymph Nodes cytology
Lymphocyte Count
Mice, Inbred C57BL
Spleen cytology
T-Lymphocytes, Regulatory metabolism
Aging, Premature immunology
Aging, Premature microbiology
B-Lymphocytes cytology
DNA-Binding Proteins metabolism
Endonucleases metabolism
Microbiota
Tryptophan metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1938-3673
- Volume :
- 101
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 27418353
- Full Text :
- https://doi.org/10.1189/jlb.1HI0216-062RR