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Increased protein expression levels of pCREB, BDNF and SDF-1/CXCR4 in the hippocampus may be associated with enhanced neurogenesis induced by environmental enrichment.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2016 Sep; Vol. 14 (3), pp. 2231-7. Date of Electronic Publication: 2016 Jul 06. - Publication Year :
- 2016
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Abstract
- Brain plasticity is very sensitive to the environment. Certain neurotrophic factors and neurotransmitter receptors, including brain-derived neurotrophic factor (BDNF), cyclic adenosine monophosphate response element‑binding protein (CREB), stromal cell‑derived factor‑1 (SDF‑1) and its specific receptor, C-X-C motif chemokine receptor 4 (CXCR4), are important in neurogenesis in adult animals. In the present study, the effects of environmental enrichment (EE) on neurogenesis in the dentate gyrus (DG) and subventricular zone (SVZ), and the protein expression levels of BDNF, CREB, SDF‑1 and CXCR4 were investigated. Adult rats were randomly assigned as controls or underwent EE for 30 days. Subsequently, immunofluorescence staining was used to analyze cell proliferation in the DG and SVZ, and the differentiation and survival of newly‑formed cells in the hippocampus. The protein expression levels of BDNF, phosphorylated CREB (pCREB), protein kinase A catalytic subunit α, SDF‑1 and CXCR4 in the hippocampus were assayed by western blotting. Cognitive function was assessed in a Morris water maze. EE improved cognitive function, and increased the proliferation, differentiation and survival of newly‑formed neurons in the DG of adult rats; however, EE did not activate neurogenesis in the SVZ. Furthermore, EE enhanced the protein expression levels of BDNF, pCREB, SDF-1 and CXCR4 in the hippocampus. These results provide a theoretical basis to explain the beneficial effects of EE on healthy, adult rats.
- Subjects :
- Animals
Biomarkers
Cell Differentiation
Cell Proliferation
Cell Survival
Cognition
Dendrites metabolism
Dentate Gyrus metabolism
Environment
Male
Maze Learning
Neurons metabolism
Rats
Brain-Derived Neurotrophic Factor metabolism
Chemokine CXCL12 metabolism
Cyclic AMP Response Element-Binding Protein metabolism
Hippocampus metabolism
Neurogenesis
Receptors, CXCR4 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 14
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 27432087
- Full Text :
- https://doi.org/10.3892/mmr.2016.5470