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Role of miR-181a-5p and endoplasmic reticulum stress in the regulation of myogenic differentiation.
- Source :
-
Gene [Gene] 2016 Oct 30; Vol. 592 (1), pp. 60-70. Date of Electronic Publication: 2016 Jul 25. - Publication Year :
- 2016
-
Abstract
- Accumulating evidence has indicated that microRNAs (miRNAs) and endoplasmic reticulum (ER) stress play critical roles in myoblast differentiation. However, the regulation roles of miRNAs and ER stress in myogenic differentiation have not been fully revealed and need to be further studied. Here, we discovered that the expression levels of miR-181a-5p were strongly upregulated during C2C12 cell differentiation. miR-181a-5p overexpression promoted ER stress and differentiation of C2C12 cells, which was accompanied by increasing expression levels of marker genes related to ER stress-mediated apoptosis and myogenic differentiation. Opposite results were observed after inhibition of the miR-181a-5p expression. The gain- and loss-of-function experiments on C2C12 cells showed that miR-181a-5p affected the development of muscle fiber type, but had no significant influence on C2C12 cell proliferation. In the ER-stressed C2C12 cells induced by thapsigargin (Tg), the expression levels of both miR-181a-5p and marker genes related to ER stress and myogenesis were upregulated. In the ER-stressed C2C12 cells and porcine muscle fibroblast (PMF) cells pretreated with Tg, we found that miR-181a-5p targeted glucose-regulated protein, 78kDa/binding immunoglobulin protein (GRP78/BIP), and influenced cell apoptosis. In conclusion, these results indicate that miR-181a-5p and ER stress have positive synergistic effects on myogenic differentiation by increasing the expression levels of myogenic differentiation key genes and activating the ER stress-mediated apoptosis signaling pathway.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Apoptosis
Cell Line
Cells, Cultured
Endoplasmic Reticulum Chaperone BiP
Gene Expression Regulation, Developmental
Heat-Shock Proteins genetics
Heat-Shock Proteins metabolism
Mice
Myoblasts cytology
Swine
Cell Differentiation
Endoplasmic Reticulum Stress
MicroRNAs genetics
Myoblasts metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0038
- Volume :
- 592
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 27461948
- Full Text :
- https://doi.org/10.1016/j.gene.2016.07.056