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Gene regulation with carbon-based siRNA conjugates for cancer therapy.
- Source :
-
Biomaterials [Biomaterials] 2016 Oct; Vol. 104, pp. 269-78. Date of Electronic Publication: 2016 Jul 19. - Publication Year :
- 2016
-
Abstract
- We report fluorescent carbon nanoparticle (FCN)-based small interfering RNA (siRNA) conjugates (C-siRNA) for gene regulation and cancer therapy. The C-siRNA has a core of chitosan-derived FCN and a shell of siRNA, and can down-regulate the expression of polo-like kinase-1 (Plk1), a master regulator of mitosis, via siRNA targeting Plk1 (siPlk1), for cancer therapy. The required amount of the FCNs is only ∼1/30 of that of the gold nanoparticles in delivering equal amount of siRNA. The C-siPlk1 led to ∼80% knockdown of cellular Plk1 mRNA in A375 cells, and induced apoptosis of the A375 cells (31.9%) and MCF-7 cells (20.33%), much higher than those by commercial nonviral gene delivery vectors, such as Lipofectamine 2000 in both cell lines (apoptosis rate < 10%). After the C-siPlk1 was administrated to A375 tumor-bearing mice intravenously, the tumor volume was less than 1/11 of the control groups. The C-siRNA can thus be powerful tools for gene delivery and gene therapy.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Gene Expression Regulation genetics
Gene Transfer Techniques
Humans
MCF-7 Cells
Nanocapsules administration & dosage
Nanocapsules chemistry
Neoplasms, Experimental pathology
Treatment Outcome
Genetic Therapy methods
Nanoconjugates administration & dosage
Nanotubes, Carbon chemistry
Neoplasms, Experimental genetics
Neoplasms, Experimental therapy
RNA, Small Interfering administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 104
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 27472164
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2016.07.015