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Arginines Plasma Concentration and Oxidative Stress in Mild to Moderate COPD.
- Source :
-
PloS one [PLoS One] 2016 Aug 01; Vol. 11 (8), pp. e0160237. Date of Electronic Publication: 2016 Aug 01 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Background: Elevated plasma concentrations of the endogenous nitric oxide synthase (NOS) inhibitor asymmetric dimethylarginine (ADMA) have been observed in respiratory conditions such as asthma and cystic fibrosis. Since oxidative stress has been shown to increase the activity of arginine methylating enzymes, hence increased ADMA synthesis, and to reduce ADMA degrading enzymes, hence increased ADMA concentrations, we assessed methylated arginines concentrations in chronic obstructive pulmonary disease (COPD), a disease characterized by increased oxidative stress.<br />Methods: Plasma arginine, ADMA and symmetric dimethylarginine (SDMA), oxidative stress markers (thiobarbituric acid reactive substances, TBARS, and plasma proteins SH, PSH) and antioxidants (taurine and paraoxonase 1, PON1, activity) were measured in 43 COPD patients with mild (n = 29) or moderate (n = 14) disease and 43 age- and sex-matched controls.<br />Results: TBARS significantly increased with COPD presence and severity (median 2.93 vs 3.18 vs 3.64 μmol/L, respectively in controls, mild and moderate group, p<0.0001 by ANOVA) whereas PSH decreased (6.69±1.15 vs 6.04±0.85 vs 5.33±0.96 μmol/gr prot, p<0.0001 by ANOVA). Increased ADMA/arginine ratio, primarily due to reduced arginine concentrations, was also observed with COPD presence and severity (median 0.0067 vs 0.0075 vs 0.0100, p<0.0001 by ANOVA). In multiple logistic regression analysis, only TBARS (OR 0.44, 95% CI 0.25-0.77; p = 0.0045) and ADMA/Arginine ratio (OR 1.72, 95% CI 2.27-13.05; p = 0.02) were independently associated with COPD severity.<br />Conclusion: COPD presence and severity are associated with increased oxidative stress and alterations in arginine metabolism. The reduced arginine concentrations in COPD may offer a new target for therapeutic interventions increasing arginine availability.
- Subjects :
- Age Factors
Aged
Aged, 80 and over
Arginine analogs & derivatives
Aryldialkylphosphatase blood
Biomarkers blood
Case-Control Studies
Female
Forced Expiratory Volume
Humans
Linear Models
Male
Middle Aged
Nitric Oxide metabolism
Pulmonary Disease, Chronic Obstructive metabolism
Severity of Illness Index
Sex Factors
Taurine blood
Arginine blood
Oxidative Stress
Pulmonary Disease, Chronic Obstructive pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 11
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 27479314
- Full Text :
- https://doi.org/10.1371/journal.pone.0160237