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MicroRNA-203 As a Stemness Inhibitor of Glioblastoma Stem Cells.
- Source :
-
Molecules and cells [Mol Cells] 2016 Aug 31; Vol. 39 (8), pp. 619-24. Date of Electronic Publication: 2016 Aug 03. - Publication Year :
- 2016
-
Abstract
- Glioblastoma stem cells (GBM-SCs) are believed to be a subpopulation within all glioblastoma (GBM) cells that are in large part responsible for tumor growth and the high grade of therapeutic resistance that is so characteristic of GBM. MicroRNAs (miR) have been implicated in regulating the expression of oncogenes and tumor suppressor genes in cancer stem cells, including GBM-SCs, and they are a potential target for cancer therapy. In the current study, miR-203 expression was reduced in CD133(+) GBM-SCs derived from six human GBM biopsies. MicroRNA-203 transfected GBM-SCs had reduced capacity for self-renewal in the cell sphere assay and increased expression of glial and neuronal differentiation markers. In addition, a reduced proliferation rate and an increased rate of apoptosis were observed. Therefore, miR-203 has the potential to reduce features of stemness, specifically in GBM-SCs, and is a logical target for GBM gene therapy.
- Subjects :
- AC133 Antigen metabolism
Adult
Apoptosis genetics
Brain Neoplasms genetics
Carcinogenesis genetics
Cell Differentiation genetics
Cell Proliferation genetics
Cell Survival genetics
Female
Gene Expression Regulation, Neoplastic
Glioblastoma genetics
Humans
Male
Middle Aged
Tumor Cells, Cultured
Brain Neoplasms therapy
Cell Self Renewal genetics
Genetic Therapy
Glioblastoma therapy
Membrane Proteins genetics
MicroRNAs genetics
Neoplastic Stem Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0219-1032
- Volume :
- 39
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Molecules and cells
- Publication Type :
- Academic Journal
- Accession number :
- 27484906
- Full Text :
- https://doi.org/10.14348/molcells.2016.0118