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Occult HBV infection in HCC and cirrhotic tissue of HBsAg-negative patients: a virological and clinical study.
- Source :
-
Oncotarget [Oncotarget] 2016 Sep 20; Vol. 7 (38), pp. 62706-62714. - Publication Year :
- 2016
-
Abstract
- Aim: To evaluate the virological and clinical characteristics of occult HBV infection (OBI) in 68 consecutive HBsAg-negative patients with biopsy-proven cirrhosis and HCC.<br />Methods: HBV DNA was sought and sequenced in plasma, HCC tissue and non-HCC liver tissue by PCRs using primers for HBV core, surface and x regions. OBI was identified by the presence of HBV DNA in at least two different PCRs.<br />Results: OBI was detected in HCC tissue of 13 (20%) patients and in non-HCC liver tissue of 3 of these 13. OBI was detected in HCC tissue of 54.5% of 11 anti-HBs- negative/anti-HBc-positive patients, in 29.4% of 17 anti-HBs/anti-HBc-positive and in 5% of 40 anti-HBs/anti-HBc-negative (p < 0.0005). The 13 patients with OBI in HCC tissue more frequently than the 55 without showed Child-B or -C cirrhosis (53.9% vs. 5.5%, p < 0.0001) and BCLC-B or -C stages (46.1% vs. 1.8%, p < 0.0001). The pre-S1, pre-S2 and S region sequences in HCC tissue showed amino acid (AA) substitutions (F19L, P24L, S59F, T131I, Q129H) and deletions (in positions 4,8, 17 and 86) in the S region, AA substitutions (T40S, P124K, L54P, G76A, N222T and I273L) in pre-S1 region and AA substitutions in pre-S2 region (P41H and P66L). In the 3 patients showing OBI also in non-HCC liver tissue the S, pre-S1 and pre-S2 sequencing displayed patterns of mutations different.<br />Conclusions: The study showed a significant correlation between OBI and the severity of liver damage, several patterns of mutations in the S, pre-S1 and pre-S2 regions in HCC tissue, some at their first description.<br />Competing Interests: All the authors of the manuscript declare that they have no conflict of interest in connection with this paper.
- Subjects :
- Aged
Biopsy
Carcinoma, Hepatocellular complications
Carcinoma, Hepatocellular genetics
DNA, Viral blood
Female
Gene Deletion
Genotype
Hepatitis B virus genetics
Humans
Liver pathology
Liver Cirrhosis complications
Liver Cirrhosis virology
Liver Neoplasms complications
Liver Neoplasms genetics
Male
Middle Aged
Mutation
Polymerase Chain Reaction
Carcinoma, Hepatocellular virology
Hepatitis B complications
Hepatitis B Surface Antigens blood
Liver Neoplasms virology
Subjects
Details
- Language :
- English
- ISSN :
- 1949-2553
- Volume :
- 7
- Issue :
- 38
- Database :
- MEDLINE
- Journal :
- Oncotarget
- Publication Type :
- Academic Journal
- Accession number :
- 27486882
- Full Text :
- https://doi.org/10.18632/oncotarget.10909