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Pathogenesis of Korean SapelovirusA in piglets and chicks.
- Source :
-
The Journal of general virology [J Gen Virol] 2016 Oct; Vol. 97 (10), pp. 2566-2574. Date of Electronic Publication: 2016 Aug 02. - Publication Year :
- 2016
-
Abstract
- Sapelovirus A (SV-A), formerly known as porcine sapelovirus as a member of a new genus Sapelovirus, is known to cause enteritis, pneumonia, polioencephalomyelitis and reproductive disorders in pigs. We have recently identified α2,3-linked sialic acid on GD1a ganglioside as a functional SV-A receptor rich in the cells of pigs and chickens. However, the role of GD1a in viral pathogenesis remains elusive. Here, we demonstrated that a Korean SV-A strain could induce diarrhoea and intestinal pathology in piglets but not in chicks. Moreover, this Korean SV-A strain had mild extra-intestinal tropisms appearing as mild, non-suppurative myelitis, encephalitis and pneumonia in piglets, but not in chicks. By real-time reverse transcription (RT) PCR, higher viral RNA levels were detected in faecal samples than in sera or extra-intestinal organs from virus-inoculated piglets. Immunohistochemistry confirmed that high viral antigens were detected in the epithelial cells of intestines from virus-inoculated piglets but not from chicks. This Korean SV-A strain could bind the cultured cell lines originated from various species, but replication occurred only in cells of porcine origin. These data indicated that this Korean SV-A strain could replicate and induce pathology in piglets but not in chicks, suggesting that additional porcine-specific factors are required for virus entry and replication. In addition, this Korean SV-A strain is enteropathogenic, but could spread to the bloodstream from the gut and disseminate to extra-intestinal organs and tissues. These results will contribute to our understanding of SV-A pathogenesis so that efficient anti-sapelovirus drugs and vaccines could be developed in the future.
- Subjects :
- Animals
Chickens
Intestines pathology
Intestines virology
Picornaviridae genetics
Picornaviridae physiology
Picornaviridae Infections pathology
Picornaviridae Infections virology
Poultry Diseases pathology
Swine
Swine Diseases pathology
Virulence
Picornaviridae pathogenicity
Picornaviridae Infections veterinary
Poultry Diseases virology
Swine Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2099
- Volume :
- 97
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 27487773
- Full Text :
- https://doi.org/10.1099/jgv.0.000571