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Prevalence and Phylogenetic Analysis of Human Bocaviruses 1-4 in Pediatric Patients with Various Infectious Diseases.
- Source :
-
PloS one [PLoS One] 2016 Aug 04; Vol. 11 (8), pp. e0160603. Date of Electronic Publication: 2016 Aug 04 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Objectives: Viral infections caused by human bocaviruses 1-4 (HBoV1-4) are more complicated than previously believed. A retrospective, large-scale study was undertaken to explore the prevalence of HBoV1-4 in pediatric patients with various infectious diseases and delineate their phylogenetic characteristics.<br />Methods: Clinical samples from four specimen types, including 4,941 respiratory, 2,239 cerebrospinal fluid (CSF), 2,619 serum, and 1,121 fecal specimens, collected from pediatric patients with various infectious diseases were screened for HBoV1-4. A 690-nt fragment in each specimen was then amplified and sequenced for phylogenetic analysis. Clinical characteristics of HBoV-positive patients with different specimen types available were evaluated.<br />Results: Approximately 1.2% of patients were confirmed as HBoV-positive, with the highest positive rate in patients with gastrointestinal infection (2.2%), followed by respiratory (1.65%), central nervous system (0.8%), and hematological infections (0.2%). A single genetic lineage of HBoV1 circulated among children over the 8-year period, while a new cluster of HBoV2, via intra-genotype recombination between HBoV2A and HBoV2B, was prevalent. Some patients had HBoV1-positive respiratory and serum specimens or fecal specimens. Several cases became HBoV1-positive following the appearance of respiratory infection, while several cases were positive for HBoV2 only in CSF and serum specimens, rather than respiratory specimens.<br />Conclusions: A single genetic lineage of HBoV1 is speculated as a viral pathogen of respiratory infection and causes both comorbid infection and acute gastroenteritis. Additionally, a new cluster of HBoV2 is prevalent in China, which may infect the host through sites other than the respiratory tract.
- Subjects :
- Adolescent
Child
Child, Preschool
DNA, Viral blood
DNA, Viral cerebrospinal fluid
DNA, Viral metabolism
Feces virology
Female
Gastroenteritis diagnosis
Gastroenteritis epidemiology
Genetic Linkage
Genotype
Human bocavirus isolation & purification
Humans
Infant
Infant, Newborn
Male
Parvoviridae Infections diagnosis
Parvoviridae Infections epidemiology
Prevalence
Respiratory Tract Infections diagnosis
Respiratory Tract Infections epidemiology
Retrospective Studies
Seasons
Gastroenteritis virology
Human bocavirus classification
Human bocavirus genetics
Parvoviridae Infections virology
Phylogeny
Respiratory Tract Infections virology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 11
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 27490242
- Full Text :
- https://doi.org/10.1371/journal.pone.0160603