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Rice (Oryza sativa) Laccases Involved in Modification and Detoxification of Herbicides Atrazine and Isoproturon Residues in Plants.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2016 Aug 24; Vol. 64 (33), pp. 6397-406. Date of Electronic Publication: 2016 Aug 15. - Publication Year :
- 2016
-
Abstract
- Atrazine (ATR) and isoproturon (IPU) as herbicides have become serious environmental contaminants due to their overuse in crop production. Although ATR and IPU in soils are easily absorbed by many crops, the mechanisms for their degradation or detoxification in plants are poorly understood. This study identified a group of novel genes encoding laccases (EC 1.10.3.2) that are possibly involved in catabolism or detoxification of ATR and IPU residues in rice. Transcriptome profiling shows at least 22 differentially expressed laccase genes in ATR/IPU-exposed rice. Some of the laccase genes were validated by RT-PCR analysis. The biochemical properties of the laccases were analyzed, and their activities in rice were induced under ATR/IPU exposure. To investigate the roles of laccases in degrading or detoxifying ATR/IPU in rice, transgenic yeast cells (Pichia pastoris X-33) expressing two rice laccase genes (LOC_Os01g63180 and LOC_Os12g15680) were generated. Both transformants were found to accumulate less ATR/IPU compared to the control. The ATR/IPU-degraded products in the transformed yeast cells using UPLC-TOF-MS/MS were further characterized. Two metabolites, hydroxy-dehydrogenated atrazine (HDHA) and 2-OH-isopropyl-IPU, catalyzed by laccases were detected in the eukaryotic cells. These results indicate that the laccase-coding genes identified here could confer degradation or detoxification of the herbicides and suggest that the laccases could be one of the important enzymatic pathways responsible for ATR/IPU degradation/detoxification in rice.
- Subjects :
- Chromatography, High Pressure Liquid
Gene Expression Profiling
Inactivation, Metabolic
Laccase genetics
Pichia genetics
Pichia metabolism
Plant Proteins genetics
Soil Pollutants metabolism
Tandem Mass Spectrometry
Transformation, Genetic
Atrazine metabolism
Herbicides metabolism
Laccase metabolism
Oryza enzymology
Phenylurea Compounds metabolism
Plant Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 64
- Issue :
- 33
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 27499219
- Full Text :
- https://doi.org/10.1021/acs.jafc.6b02187