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Mitochondria-Mediated Protein Regulation Mechanism of Polymorphs-Dependent Inhibition of Nanoselenium on Cancer Cells.
- Source :
-
Scientific reports [Sci Rep] 2016 Aug 12; Vol. 6, pp. 31427. Date of Electronic Publication: 2016 Aug 12. - Publication Year :
- 2016
-
Abstract
- The present study was (i) to prepare two types of selenium nanoparticles, namely an amorphous form of selenium quantum dots (A-SeQDs) and a crystalline form of selenium quantum dots (C-SeQDs); and (ii) to investigate the nano-bio interactions of A-SeQDs and C-SeQDs in MCF-7, HepG2, HeLa, NIH/3T3, L929 cells and BRL-3A cells. It was found that A-SeQDs could induce the mitochondria-mediated apoptosis, necrosis and death of cells, while C-SeQDs had much weaker effects. This polymorphs-dependent anti-proliferative activity of nano-selenium was scarcely reported. Further investigation demonstrated that A-SeQDs could differentially regulate 61 proteins and several pathways related to stress response, protein synthesis, cell migration and cell cycle, including "p38 MAPK Signaling", "p53 Signaling", "14-3-3-mediated Signaling", "p70S6K Signaling" and "Protein Ubiquitination Pathway". This was the first report to demonstrate the involvement of protein synthesis and post-translational modification pathways in the anti-proliferative activity associated with NMs. Compared with previously fragmentary studies, this study use a nanomics approach combining bioinformatics and proteomics to systematically investigate the nano-bio interactions of selenium nanoparticles in cancer cells.
- Subjects :
- Animals
Apoptosis drug effects
Cell Cycle Checkpoints drug effects
Cell Proliferation drug effects
Endoplasmic Reticulum Chaperone BiP
HeLa Cells
Heat-Shock Proteins metabolism
Humans
MCF-7 Cells
Membrane Potential, Mitochondrial drug effects
Mice
Microscopy, Confocal
Mitochondria drug effects
NIH 3T3 Cells
Protein Disulfide-Isomerases metabolism
Quantum Dots chemistry
Quantum Dots toxicity
Signal Transduction drug effects
Thioredoxin Reductase 1 metabolism
Mitochondria metabolism
Mitochondrial Proteins metabolism
Quantum Dots metabolism
Selenium chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27514819
- Full Text :
- https://doi.org/10.1038/srep31427