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Nanocurcumin accords protection against acute hypobaric hypoxia induced lung injury in rats.
- Source :
-
Journal of physiology and biochemistry [J Physiol Biochem] 2016 Dec; Vol. 72 (4), pp. 763-779. Date of Electronic Publication: 2016 Aug 17. - Publication Year :
- 2016
-
Abstract
- Decline in oxygen availability experienced under hypobaric hypoxia (HH) mediates imbalance in lung fluid clearance and is a causative agent of acute lung injury. Here, we investigate the pathological events behind acute HH mediated lung injury and assess the therapeutic efficacy of nanocurcumin in its amelioration. We assess the protective efficacy of nanotized curcumin (nanocurcumin) in ameliorating HH induced lung injury and compare to curcumin. Rats exposed to acute HH (6, 12, 24, 48 and 72 h) were subjected to histopathology, blood-gas analysis and clinical biochemistry, cytokine response and redox damage. HH induced lung injury was analysed using markers of lung injury due to pulmonary vasoconstriction (ET-1/2/3 and endothelin receptors A and B) and trans-vascular fluid balance mediator (Na+/K+ ATPase). The protective efficacy of nanocurcumin was analysed by examination of Akt/Erk signalling cascade by western blot. HH induced lung injury was associated with discrete changes in blood analytes, differential circulatory cytokine response and severe pulmonary redox damages. Up-regulation of ET-1/2/3 and its receptors along with down-regulation of Na+/K+ ATPase confirmed defective pulmonary fluid clearance which promoted edema formation. Nanocurcumin treatment prevented lung edema formation and restored expression levels of ET-1/2/3 and its receptors while restoring the blood analytes, circulatory cytokines and pulmonary redox status better than curcumin. Modulation in Akt/Erk signalling pathway in rat lungs under HH confirmed the protective efficacy of nanocurcumin.
- Subjects :
- Acute Lung Injury etiology
Acute Lung Injury genetics
Acute Lung Injury pathology
Animals
Biomarkers metabolism
Disease Models, Animal
Endothelin-1 genetics
Endothelin-1 metabolism
Endothelin-2 genetics
Endothelin-2 metabolism
Endothelin-3 genetics
Endothelin-3 metabolism
Hypoxia complications
Hypoxia genetics
Hypoxia pathology
Male
Mitogen-Activated Protein Kinase 1 genetics
Mitogen-Activated Protein Kinase 1 metabolism
Mitogen-Activated Protein Kinase 3 genetics
Mitogen-Activated Protein Kinase 3 metabolism
Nanostructures chemistry
Oxidation-Reduction drug effects
Proto-Oncogene Proteins c-akt genetics
Proto-Oncogene Proteins c-akt metabolism
Rats
Rats, Sprague-Dawley
Receptor, Endothelin A genetics
Receptor, Endothelin A metabolism
Receptor, Endothelin B genetics
Receptor, Endothelin B metabolism
Signal Transduction
Sodium-Potassium-Exchanging ATPase genetics
Sodium-Potassium-Exchanging ATPase metabolism
Acute Lung Injury drug therapy
Curcumin pharmacology
Gene Expression Regulation drug effects
Hypoxia drug therapy
Nanostructures therapeutic use
Protective Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1877-8755
- Volume :
- 72
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of physiology and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 27534650
- Full Text :
- https://doi.org/10.1007/s13105-016-0515-3