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Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells.
- Source :
-
Marine drugs [Mar Drugs] 2016 Aug 16; Vol. 14 (8). Date of Electronic Publication: 2016 Aug 16. - Publication Year :
- 2016
-
Abstract
- In this study, the hexapeptide Phe-Ile-Met-Gly-Pro-Tyr (FIMGPY), which has a molecular weight of 726.9 Da, was separated from skate (Raja porosa) cartilage protein hydrolysate using ultrafiltration and chromatographic methods, and its anticancer activity was evaluated in HeLa cells. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay indicated that FIMGPY exhibited high, dose-dependent anti-proliferation activities in HeLa cells with an IC50 of 4.81 mg/mL. Acridine orange/ethidium bromide (AO/EB) fluorescence staining and flow cytometry methods confirmed that FIMGPY could inhibit HeLa cell proliferation by inducing apoptosis. Western blot assay revealed that the Bax/Bcl-2 ratio and relative intensity of caspase-3 in HeLa cells treated with 7-mg/mL FIMGPY were 2.63 and 1.83, respectively, significantly higher than those of the blank control (p < 0.01). Thus, FIMGPY could induce apoptosis by upregulating the Bax/Bcl-2 ratio and caspase-3 activation. Using a DNA ladder method further confirmed that the anti-proliferation activity of FIMGPY was attributable to its role in inducing apoptosis. These results suggest that FIMGPY from skate cartilage protein hydrolysate may have applications as functional foods and nutraceuticals for the treatment and prevention of cancer.
- Subjects :
- Animals
Antineoplastic Agents isolation & purification
Caspase 3 metabolism
Cell Proliferation drug effects
Dietary Supplements
Female
Flow Cytometry
HeLa Cells
Humans
Inhibitory Concentration 50
Neoplasms prevention & control
Oligopeptides isolation & purification
Protein Hydrolysates isolation & purification
Proto-Oncogene Proteins c-bcl-2 metabolism
Ultrafiltration
Up-Regulation
bcl-2-Associated X Protein metabolism
Antineoplastic Agents pharmacology
Apoptosis drug effects
Cartilage chemistry
Oligopeptides pharmacology
Protein Hydrolysates chemistry
Skates, Fish
Subjects
Details
- Language :
- English
- ISSN :
- 1660-3397
- Volume :
- 14
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Marine drugs
- Publication Type :
- Academic Journal
- Accession number :
- 27537897
- Full Text :
- https://doi.org/10.3390/md14080153