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Stimulated Suicidal Erythrocyte Death in Arteritis.
- Source :
-
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2016; Vol. 39 (3), pp. 1068-77. Date of Electronic Publication: 2016 Aug 22. - Publication Year :
- 2016
-
Abstract
- Background/aims: Arteritis is an inflammatory disease of the vascular wall leading to ischemia and vascular occlusion. Complications of arteritis include anemia, which could, at least in theory, result from suicidal erythrocyte death or eryptosis, which is characterized by erythrocyte shrinkage and phosphatidylserine (PS) exposure at the erythrocyte surface. Cellular mechanisms involved in the stimulation of eryptosis include increased cytosolic Ca2+-concentration ([Ca2+]i), oxidative stress and ceramide formation. The present study explored whether and how arteritis influences eryptosis.<br />Methods: Blood was drawn from patients suffering from arteritis (n=17) and from healthy volunteers (n=21). PS exposure was estimated from annexin V-binding, erythrocyte volume from forward scatter, [Ca2+]i from Fluo3-fluorescence, reactive oxygen species (ROS) from DCFDA fluorescence and ceramide abundance from FITC-conjugated antibody binding in flow cytometry. The patients suffered from anemia despite 2.8±0.4% reticulocytes.<br />Results: The percentage of PS-exposing erythrocytes was significantly higher in patients (1.1±0.1%) than in healthy volunteers (0.3±0.1%). The increase in PS exposure was paralleled by increase in oxidative stress and [Ca2+]i but not by significant changes of ceramide abundance. Erythrocyte PS exposure and ROS production were significantly enhanced in erythrocytes exposed to patient plasma as compared to exposure to plasma from healthy volunteers.<br />Conclusion: Arteritis is associated with enhanced eryptosis due to increased [Ca2+]i and oxidative stress. The eryptosis contributes to or even accounts for the anemia in those patients. As eryptotic erythrocytes adhere to endothelial cells of the vascular wall, they could impede microcirculation and thus contribute to vascular occlusion.<br /> (© 2016 The Author(s) Published by S. Karger AG, Basel.)
- Subjects :
- Aged
Anemia complications
Anemia pathology
Aniline Compounds
Annexin A5
Arteritis complications
Arteritis pathology
Case-Control Studies
Ceramides blood
Erythrocytes metabolism
Erythrocytes pathology
Female
Flow Cytometry
Fluoresceins
Fluorescent Dyes
Humans
Male
Middle Aged
Primary Cell Culture
Reactive Oxygen Species blood
Xanthenes
Anemia blood
Arteritis blood
Calcium blood
Eryptosis
Oxidative Stress
Phosphatidylserines blood
Subjects
Details
- Language :
- English
- ISSN :
- 1421-9778
- Volume :
- 39
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 27544243
- Full Text :
- https://doi.org/10.1159/000447814