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Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers.
- Source :
-
International journal of nanomedicine [Int J Nanomedicine] 2016 Aug 17; Vol. 11, pp. 3927-37. Date of Electronic Publication: 2016 Aug 17 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- In this study, we developed biodegradable sheath-core-structured drug-eluting nanofibers for sustainable delivery of antibiotics (vancomycin and ceftazidime) and recombinant human bone morphogenetic protein (rhBMP-2) via electrospinning. To prepare the biodegradable sheath-core nanofibers, we first prepared solutions of poly(d,l)-lactide-co-glycolide, vancomycin, and ceftazidime in 1,1,1,3,3,3-hexafluoro-2-propanol and rhBMP-2 in phosphate-buffered solution. The poly(d,l)-lactide-co-glycolide/antibiotics and rhBMP-2 solutions were then fed into two different capillary tubes controlled by two independent pumps for coaxial electrospinning. The electrospun nanofiber morphology was observed under a scanning electron microscope. We further characterized the in vitro antibiotic release from the nanofibers via high-performance liquid chromatography and that of rhBMP-2 via enzyme-linked immunosorbent assay and alkaline phosphatase activity. We showed that the biodegradable coaxially electrospun nanofibers could release high vancomycin/ceftazidime concentrations (well above the minimum inhibition concentration [MIC]90) and rhBMP-2 for >4 weeks. These experimental results demonstrate that novel biodegradable nanofibers can be constructed with various pharmaceuticals and proteins for long-term drug deliveries.
- Subjects :
- Animals
Ceftazidime pharmacology
Drug Liberation
Humans
Microbial Sensitivity Tests
Nanofibers ultrastructure
Polylactic Acid-Polyglycolic Acid Copolymer
Rats, Sprague-Dawley
Spectroscopy, Fourier Transform Infrared
Tensile Strength
Vancomycin pharmacology
Water chemistry
Anti-Bacterial Agents pharmacology
Biocompatible Materials chemistry
Bone Morphogenetic Protein 2 pharmacology
Drug Delivery Systems
Lactic Acid chemistry
Nanofibers chemistry
Polyglycolic Acid chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1178-2013
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 27574423
- Full Text :
- https://doi.org/10.2147/IJN.S107250