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Two spatially and temporally distinct Ca 2+ signals convey Arabidopsis thaliana responses to K + deficiency.
- Source :
-
The New phytologist [New Phytol] 2017 Jan; Vol. 213 (2), pp. 739-750. Date of Electronic Publication: 2016 Aug 31. - Publication Year :
- 2017
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Abstract
- In plants, potassium (K <superscript>+</superscript> ) homeostasis is tightly regulated and established against a concentration gradient to the environment. Despite the identification of Ca <superscript>2+</superscript> -regulated kinases as modulators of K <superscript>+</superscript> channels, the immediate signaling and adaptation mechanisms of plants to low-K <superscript>+</superscript> conditions are only partially understood. To assess the occurrence and role of Ca <superscript>2+</superscript> signals in Arabidopsis thaliana roots, we employed ratiometric analyses of Ca <superscript>2+</superscript> dynamics in plants expressing the Ca <superscript>2+</superscript> reporter YC3.6 in combination with patch-clamp analyses of root cells and two-electrode voltage clamp (TEVC) analyses in Xenopus laevis oocytes. K <superscript>+</superscript> deficiency triggers two successive and distinct Ca <superscript>2+</superscript> signals in roots exhibiting spatial and temporal specificity. A transient primary Ca <superscript>2+</superscript> signature arose within 1 min in the postmeristematic stelar tissue of the elongation zone, while a secondary Ca <superscript>2+</superscript> response occurred after several hours as sustained Ca <superscript>2+</superscript> elevation in defined tissues of the elongation and root hair differentiation zones. Patch-clamp and TEVC analyses revealed Ca <superscript>2+</superscript> dependence of the activation of the K <superscript>+</superscript> channel AKT1 by the CBL1-CIPK23 Ca <superscript>2+</superscript> sensor-kinase complex. Together, these findings identify a critical role of cell group-specific Ca <superscript>2+</superscript> signaling in low K <superscript>+</superscript> responses and indicate an essential and direct role of Ca <superscript>2+</superscript> signals for AKT1 K <superscript>+</superscript> channel activation in roots.<br /> (© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.)
- Subjects :
- Adaptation, Physiological drug effects
Animals
Arabidopsis drug effects
Arabidopsis Proteins chemistry
Arabidopsis Proteins metabolism
Calcium metabolism
Cytoplasm drug effects
Cytoplasm metabolism
Cytosol drug effects
Cytosol metabolism
Electrodes
Ion Channel Gating drug effects
Lanthanum pharmacology
Mutation genetics
Oocytes drug effects
Oocytes metabolism
Patch-Clamp Techniques
Plant Roots cytology
Plant Roots drug effects
Plant Roots metabolism
Protein Domains
Protoplasts drug effects
Protoplasts metabolism
Time Factors
Xenopus
Arabidopsis metabolism
Calcium Signaling drug effects
Potassium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 213
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 27579668
- Full Text :
- https://doi.org/10.1111/nph.14145