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Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer.

Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer.

Authors :
Sagiv JY
Voels S
Granot Z
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1458, pp. 179-93.
Publication Year :
2016

Abstract

Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years accumulating evidence has suggested that neutrophils are heterogeneous and that distinct neutrophil subsets may play very different roles. Here, we describe the methodology for isolation of high- and low-density neutrophils from the murine and human circulation using a density gradient and antibody based enrichment. We further describe the methodology for functional characterization of these different neutrophil subsets in the context of cancer.

Details

Language :
English
ISSN :
1940-6029
Volume :
1458
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
27581022
Full Text :
https://doi.org/10.1007/978-1-4939-3801-8_13