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Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer.
Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1458, pp. 179-93. - Publication Year :
- 2016
-
Abstract
- Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years accumulating evidence has suggested that neutrophils are heterogeneous and that distinct neutrophil subsets may play very different roles. Here, we describe the methodology for isolation of high- and low-density neutrophils from the murine and human circulation using a density gradient and antibody based enrichment. We further describe the methodology for functional characterization of these different neutrophil subsets in the context of cancer.
- Subjects :
- Animals
Biomarkers
Cell Separation
Chemotaxis, Leukocyte immunology
Cytotoxicity, Immunologic
Disease Models, Animal
Humans
Immunophenotyping
Neutrophils metabolism
Phagocytosis
Phenotype
Respiratory Burst immunology
Neoplasms immunology
Neoplasms pathology
Neutrophil Infiltration immunology
Neutrophils immunology
Neutrophils pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1458
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 27581022
- Full Text :
- https://doi.org/10.1007/978-1-4939-3801-8_13