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Effects of cytochalasin B on DNA methylation and histone modification in parthenogenetically activated porcine embryos.
- Source :
-
Reproduction (Cambridge, England) [Reproduction] 2016 Nov; Vol. 152 (5), pp. 519-27. Date of Electronic Publication: 2016 Aug 31. - Publication Year :
- 2016
-
Abstract
- DNA methylation and histone modification play important roles in the development of mammalian embryos. Cytochalasin B (CB) is an actin polymerization inhibitor that can significantly affect cell activity and is often used in studies concerning cytology. In recent years, CB is also commonly being used in in vitro experiments on mammalian embryos, but few studies have addressed the effect of CB on the epigenetic modification of embryonic development, and the mechanism underlying this process is also unknown. This study was conducted to investigate the effects of CB on DNA methylation and histone modification in the development of parthenogenetically activated porcine embryos. Treatment with 5 μg/mL CB for 4 h significantly increased the cleavage rate, blastocyst rate and total cell number of blastocysts. However, the percentage of apoptotic cells and the expression levels of the apoptosis-related genes BCL-XL, BAX and CASP3 were significantly decreased. Treatment with CB significantly decreased the expression levels of DNMT1, DNMT3a, DNMT3b, HAT1 and HDAC1 at the pronuclear stage and promoted the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). After CB treatment, the level of AcH3K9 was upregulated and the level of H3K9me3 was downregulated. When combined with Scriptaid and 5-Aza-Cdr, CB further improved the embryonic development competence and decreased the expression of BCL-XL, BAX and CASP3 In conclusion, these results suggest that CB could improve embryonic development and the quality of the blastocyst by improving the epigenetic modification during the development of parthenogenetically activated embryos.<br /> (© 2016 Society for Reproduction and Fertility.)
- Subjects :
- Animals
Apoptosis drug effects
Blastocyst drug effects
Blastocyst metabolism
DNA (Cytosine-5-)-Methyltransferases metabolism
Embryo, Mammalian drug effects
Embryo, Mammalian metabolism
Epigenesis, Genetic
Female
Histone Deacetylase Inhibitors pharmacology
Histones metabolism
Nuclear Transfer Techniques
Pregnancy
Swine
DNA Methyltransferase 3B
Blastocyst cytology
Cytochalasin B pharmacology
DNA Methylation drug effects
Embryo, Mammalian cytology
Embryonic Development drug effects
Histones chemistry
Parthenogenesis
Subjects
Details
- Language :
- English
- ISSN :
- 1741-7899
- Volume :
- 152
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Reproduction (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 27581081
- Full Text :
- https://doi.org/10.1530/REP-16-0280