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Single-Tube Flow Cytometry Assay for the Detection of Mature Lymphoid Neoplasms in Paucicellular Samples.
- Source :
-
Acta cytologica [Acta Cytol] 2016; Vol. 60 (4), pp. 385-394. Date of Electronic Publication: 2016 Sep 07. - Publication Year :
- 2016
-
Abstract
- Objectives: Flow cytometry (FC) has become a useful support for cytomorphologic evaluation (CM) of fine-needle aspirates (FNA) and serous cavity effusions (SCE) in cases of suspected non-Hodgkin lymphoma (NHL). FC results may be hampered by the scarce viability and low cellularity of the specimens.<br />Study Design: We developed a single-tube FC assay (STA) that included 10 antibodies cocktailed in 8-color labeling, a cell viability dye, and a logical gating strategy to detect NHL in hypocellular samples. The results were correlated with CM and confirmed by histologic or molecular data when available.<br />Results: Using the STA, we detected B-type NHL in 31 out of 103 hypocellular samples (81 FNA and 22 SCE). Of these, 8 were not confirmed by CM and 2 were considered to be only suspicious. The FC-negative samples had a final diagnosis of benign/reactive process (42/72), carcinoma (27/72), or Hodgkin lymphoma (3/72).<br />Conclusions: The STA approach allowed obtainment of maximum immunophenotyping data in specimens containing a low number of cells and a large amount of debris. The information obtained by STA can help cytomorphologists not only to recognize but also to exclude malignant lymphomas.<br /> (© 2016 S. Karger AG, Basel.)
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Biopsy, Fine-Needle methods
Humans
Immunophenotyping methods
Middle Aged
Young Adult
Carcinoma diagnosis
Carcinoma pathology
Flow Cytometry methods
Hodgkin Disease diagnosis
Hodgkin Disease pathology
Lymphoma, Non-Hodgkin diagnosis
Lymphoma, Non-Hodgkin pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1938-2650
- Volume :
- 60
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Acta cytologica
- Publication Type :
- Academic Journal
- Accession number :
- 27597993
- Full Text :
- https://doi.org/10.1159/000448799