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Endothelin-1 Treatment Induces an Experimental Cerebral Malaria-Like Syndrome in C57BL/6 Mice Infected with Plasmodium berghei NK65.
- Source :
-
The American journal of pathology [Am J Pathol] 2016 Nov; Vol. 186 (11), pp. 2957-2969. Date of Electronic Publication: 2016 Sep 15. - Publication Year :
- 2016
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Abstract
- Plasmodium berghei ANKA infection of C57BL/6 mice is a widely used model of experimental cerebral malaria (ECM). By contrast, the nonneurotropic P. berghei NK65 (PbN) causes severe malarial disease in C57BL/6 mice but does not cause ECM. Previous studies suggest that endothelin-1 (ET-1) contributes to the pathogenesis of ECM. In this study, we characterize the role of ET-1 on ECM vascular dysfunction. Mice infected with 10 <superscript>6</superscript> PbN-parasitized red blood cells were treated with either ET-1 or saline from 2 to 8 days postinfection (dpi). Plasmodium berghei ANKA-infected mice served as the positive control. ET-1-treated PbN-infected mice exhibited neurological signs, hypothermia, and behavioral alterations characteristic of ECM, dying 4 to 8 dpi. Parasitemia was not affected by ET-1 treatment. Saline-treated PbN-infected mice did not display ECM, surviving until 12 dpi. ET-1-treated PbN-infected mice displayed leukocyte adhesion to the vascular endothelia and petechial hemorrhages throughout the brain at 6 dpi. Intravital microscopic images demonstrated significant brain arteriolar vessel constriction, decreased functional capillary density, and increased blood-brain barrier permeability. These alterations were not present in either ET-1-treated uninfected or saline-treated PbN-infected mice. In summary, ET-1 treatment of PbN-infected mice induced an ECM-like syndrome, causing brain vasoconstriction, adherence of activated leukocytes in the cerebral microvasculature, and blood-brain barrier leakage, indicating that ET-1 is involved in the genesis of brain microvascular alterations that are the hallmark of ECM.<br /> (Copyright © 2016 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Blood-Brain Barrier drug effects
Brain pathology
Cell Adhesion
Disease Models, Animal
Endothelin-1 therapeutic use
Endothelium, Vascular pathology
Female
Leukocytes pathology
Malaria, Cerebral drug therapy
Mice
Mice, Inbred C57BL
Parasitemia
Endothelin-1 adverse effects
Malaria, Cerebral pathology
Plasmodium berghei physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1525-2191
- Volume :
- 186
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 27640146
- Full Text :
- https://doi.org/10.1016/j.ajpath.2016.07.020