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Magnetic resonance imaging of osteosarcoma using a bis(alendronate)-based bone-targeted contrast agent.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2016 Dec; Vol. 84, pp. 423-429. Date of Electronic Publication: 2016 Sep 25. - Publication Year :
- 2016
-
Abstract
- Magnetic resonance (MR) is currently used for diagnosis of osteosarcoma but not well even though contrast agents are administered. Here, we report a novel bone-targeted MR imaging contrast agent, Gd <subscript>2</subscript> -diethylenetriaminepentaacetate-bis(alendronate) (Gd <subscript>2</subscript> -DTPA-BA) for the diagnosis of osteosarcoma. It is the conjugate of a bone cell-seeking molecule (i.e., alendronate) and an MR imaging contrast agent (i.e., Gd-DTPA). Its physicochemical parameters were measured, including pK <subscript>a</subscript> , complex constant, and T <subscript>1</subscript> relaxivity. Its bone cell-seeking ability was evaluated by measuring its adsorption on hydroxyapatite. Hemolysis was investigated. MR imaging and biodistribution of Gd <subscript>2</subscript> -DTPA-BA and Gd-DTPA were studied on healthy and osteosarcoma-bearing nude mice. Gd <subscript>2</subscript> -DTPA-BA showed high adsorption on hydroxyapatite, the high MR relaxivity (r <subscript>1</subscript> ) of 7.613mM <superscript>-1</superscript> s <superscript>-1</superscript> (2.6 folds of Gd-DTPA), and no hemolysis. The MR contrast effect of Gd <subscript>2</subscript> -DTPA-BA was much higher than that of Gd-DTPA after intravenous injection to the mice. More importantly, the MR imaging of osteosarcoma was significantly improved by Gd <subscript>2</subscript> -DTPA-BA. The signal intensity of Gd <subscript>2</subscript> -DTPA-BA reached 120.3% at 50min, equal to three folds of Gd-DTPA. The bone targeting index (bone/blood) of Gd <subscript>2</subscript> -DTPA-BA in the osteosarcoma-bearing mice was very high to 130 at 180min. Furthermore, the contrast enhancement could also be found in the lung due to metastasis of osteosarcoma. Gd <subscript>2</subscript> -DTPA-BA plays a promising role in the diagnoses of osteosacomas, including the primary bone tumors and metastases.<br /> (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Animals
Biocompatible Materials pharmacology
Bone and Bones drug effects
Durapatite chemistry
Gadolinium DTPA chemistry
Macaca mulatta
Male
Mice, Inbred BALB C
Mice, Nude
Signal Processing, Computer-Assisted
Tissue Distribution drug effects
Alendronate chemistry
Bone and Bones pathology
Contrast Media chemistry
Magnetic Resonance Imaging methods
Osteosarcoma diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 84
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 27682736
- Full Text :
- https://doi.org/10.1016/j.biopha.2016.09.061