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Oral administration of antioxidants improves skin wound healing in diabetic mice.
- Source :
-
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society [Wound Repair Regen] 2016 Nov; Vol. 24 (6), pp. 981-993. Date of Electronic Publication: 2016 Oct 12. - Publication Year :
- 2016
-
Abstract
- Oxidative stress aggravates several long-term complications in diabetes mellitus. We evaluated the effectiveness of the oral administration of antioxidants (vitamins E and C, 40 and 100 mg/kg b.w., respectively) on skin wound healing acceleration in alloxan-induced diabetic mice. Mice were wounded 30 days after the induction of diabetes. Antioxidants were effective in preventing oxidative stress, as assessed by TBARS. The enzymes catalase, glutathione reductase, glutathione peroxidase, and superoxide dismutase were increased in diabetics on the 3rd day post-wounding; catalase and glutathione peroxidase remained still augmented in diabetics after 14th day postwounding, and the treatment with vitamins restored their activities to control. After 3 days, diabetic mice showed lower infiltration of inflammatory cells (including CD11b <superscript>+</superscript> and Ly6G <superscript>+</superscript> cells) and reduced levels of KC, TNF-α, IL-1β, and IL-12 p40 when compared with control mice. The treatment restored cytokine levels. After 14 days, diabetic mice showed late wound closure, persistent inflammation and delayed reepithelialization, accompanied by an increase in MIG <superscript>+</superscript> /CD206 <superscript>-</superscript> macrophages whereas CD206 <superscript>+</superscript> /MIG <superscript>-</superscript> macrophages were decreased. Cytokines IL-12p40, TNF-α, IL-1β, and KC were increased and normal levels were restored after treatment with antioxidants. These results suggest that oxidative stress plays a major role in diabetic wound healing impairment and the oral administration of antioxidants improves healing by modulating inflammation and the antioxidant system with no effect on glycemia.<br /> (© 2016 by the Wound Healing Society.)
- Subjects :
- Administration, Oral
Animals
Blood Glucose metabolism
Catalase metabolism
Interleukin-12 Subunit p40 metabolism
Mice
Superoxide Dismutase metabolism
Tumor Necrosis Factor-alpha pharmacology
Antioxidants administration & dosage
Antioxidants pharmacology
Diabetes Mellitus, Experimental pathology
Inflammation pathology
Oxidative Stress drug effects
Wound Healing drug effects
Wounds and Injuries pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1524-475X
- Volume :
- 24
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
- Publication Type :
- Academic Journal
- Accession number :
- 27684945
- Full Text :
- https://doi.org/10.1111/wrr.12486