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Biomimetic HDL nanoparticle mediated tumor targeted delivery of indocyanine green for enhanced photodynamic therapy.
- Source :
-
Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2016 Dec 01; Vol. 148, pp. 533-540. Date of Electronic Publication: 2016 Sep 26. - Publication Year :
- 2016
-
Abstract
- Photodynamic therapy has emerged as a promising strategy for cancer treatment. To ensure the efficient delivery of a photosensitizer to tumor for anticancer effect, a safe and tumor-specific delivery system is highly desirable. Herein, we introduce a novel biomimetic nanoparticle named rHDL/ICG (rHDL/I), by loading amphiphilic near-infrared (NIR) fluorescent dye indocyanine green (ICG) into reconstituted high density lipoproteins (rHDL). In this system, rHDL can mediate photoprotection effect and receptor-guided tumor-targeting transportation of cargos into cells. Upon NIR irradiation, ICG can generate fluorescent imaging signals for diagnosis and monitoring therapeutic activity, and produce singlet oxygen to trigger photodynamic therapy (PDT). Our studies demonstrated that rHDL/I exhibited excellent size and fluorescence stability, light-triggered controlled release feature, and neglectable hemolytic activity. It also showed equivalent NIR response compared to free ICG under laser irradiation. Importantly, the fluorescent signal of ICG loaded in rHDL/I could be visualized subcellularly in vitro and exhibited metabolic distribution in vivo, presenting superior tumor targeting and internalization. This NIR-triggered image-guided nanoparticle produced outstanding therapeutic outcomes against cancer cells, demonstrating great potential of biomimetic delivery vehicles in future clinical practice.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Biomimetic Materials administration & dosage
Biomimetic Materials chemistry
Biomimetic Materials pharmacokinetics
Carcinoma, Hepatocellular metabolism
Carcinoma, Hepatocellular pathology
Cell Survival drug effects
Drug Liberation
Drug Stability
Female
Hemolysis drug effects
Hep G2 Cells
Humans
Indocyanine Green chemistry
Indocyanine Green pharmacokinetics
Infrared Rays
Lipoproteins, HDL chemistry
Lipoproteins, HDL pharmacokinetics
Liver Neoplasms metabolism
Liver Neoplasms pathology
Mice, Inbred BALB C
Mice, Nude
Microscopy, Confocal
Microscopy, Electron, Transmission
Nanoparticles chemistry
Nanoparticles ultrastructure
Rabbits
Reactive Oxygen Species metabolism
Transplantation, Heterologous
Drug Delivery Systems methods
Indocyanine Green administration & dosage
Lipoproteins, HDL administration & dosage
Nanoparticles administration & dosage
Photochemotherapy methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4367
- Volume :
- 148
- Database :
- MEDLINE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 27690242
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2016.09.037