Back to Search
Start Over
Increased detection of Dirofilaria immitis antigen in cats after heat pretreatment of samples.
- Source :
-
Journal of feline medicine and surgery [J Feline Med Surg] 2017 Oct; Vol. 19 (10), pp. 1013-1016. Date of Electronic Publication: 2016 Sep 30. - Publication Year :
- 2017
-
Abstract
- Objectives To determine whether pretreating diagnostic samples with heat increases the detection of Dirofilaria immitis antigen in adult cats, we evaluated feline serum and plasma samples collected in heartworm-endemic areas of the southern United States. Methods Commercial microtiter well assays for detection of D immitis antigen were used to evaluate serum or plasma samples from 385 shelter and free-roaming cats from the southcentral and southeastern United States before and after heat treatment; commercial antibody tests were performed on a subset of samples. Results Prior to sample heat treatment, 1/220 (0.5%) shelter cats and 4/165 (2.4%) free-roaming cats had detectable D immitis antigen. After heat pretreatment, the detection rate increased to 13/220 (5.9%) and 13/165 (7.9%), respectively. Antibody reactive to D immitis was significantly more common ( P <0.001) in the serum of cats that were antigen positive after heat treatment (10/13; 76.9%) than serum from cats that remained antigen negative after heat treatment (22/163; 13.5%). Conclusions and relevance Heat pretreatment of feline samples increased antigen detection by commercial assays for D immitis and improved overall concordance of antigen and antibody test results in antigen-positive samples in this population. Although further work to investigate the specificity of D immitis antigen assays when using pre-treated samples is warranted, this approach may be useful in the diagnosis of heartworm infection in individual cats and may increase the accuracy of surveys based on antigen detection.
- Subjects :
- Animals
Animals, Wild
Cat Diseases blood
Cat Diseases diagnosis
Cats
Dirofilaria immitis immunology
Dirofilariasis blood
Dirofilariasis parasitology
Hot Temperature
Specimen Handling methods
Specimen Handling veterinary
Antigens, Helminth blood
Cat Diseases parasitology
Dirofilaria immitis isolation & purification
Dirofilariasis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2750
- Volume :
- 19
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of feline medicine and surgery
- Publication Type :
- Academic Journal
- Accession number :
- 27694369
- Full Text :
- https://doi.org/10.1177/1098612X16670562