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Optimization of Early Response Monitoring and Prediction of Cancer Antiangiogenesis Therapy via Noninvasive PET Molecular Imaging Strategies of Multifactorial Bioparameters.
- Source :
-
Theranostics [Theranostics] 2016 Sep 10; Vol. 6 (12), pp. 2084-2098. Date of Electronic Publication: 2016 Sep 10 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Objective : Antiangiogenesis therapy (AAT) has provided substantial benefits regarding improved outcomes and survival for suitable patients in clinical settings. Therefore, the early definition of therapeutic effects is urgently needed to guide cancer AAT. We aimed to optimize the early response monitoring and prediction of AAT efficacy, as indicated by the multi-targeted anti-angiogenic drug sunitinib in U87MG tumors, using noninvasive positron emission computed tomography (PET) molecular imaging strategies of multifactorial bioparameters. Methods: U87MG tumor mice were treated via intragastric injections of sunitinib (80 mg/kg) or vehicle for 7 consecutive days. Longitudinal MicroPET/CT scans with <superscript>18</superscript> F-FDG, <superscript>18</superscript> F-FMISO, <superscript>18</superscript> F-ML-10 and <superscript>18</superscript> F-Alfatide II were acquired to quantitatively measure metabolism, hypoxia, apoptosis and angiogenesis on days 0, 1, 3, 7 and 13 following therapy initiation. Tumor tissues from a dedicated group of mice were collected for immunohistochemical (IHC) analysis of key biomarkers (Glut-1, CA-IX, TUNEL, α <subscript>ν</subscript> β <subscript>3</subscript> and CD31) at the time points of PET imaging. The tumor sizes and mouse weights were measured throughout the study. The tumor uptake (ID%/g <subscript>max</subscript> ), the ratios of the tumor/muscle (T/M) for each probe, and the tumor growth ratios (TGR) were calculated and used for statistical analyses of the differences and correlations. Results: Sunitinib successfully inhibited U87MG tumor growth with significant differences in the tumor size from day 9 after sunitinib treatment compared with the control group (P < 0.01). The uptakes of <superscript>18</superscript> F-FMISO (reduced hypoxia), <superscript>18</superscript> F-ML-10 (increased apoptosis) and <superscript>18</superscript> F-Alfatide II (decreased angiogenesis) in the tumor lesions significantly changed during the early stage (days 1 to 3) of sunitinib treatment; however, the uptake of <superscript>18</superscript> F-FDG (increased glucose metabolism) was significantly different during the late stage. The PET imaging data of each probe were all confirmed via ex vivo IHC of the relevant biomarkers. Notably, the PET imaging of <superscript>18</superscript> F-Alfatide II and <superscript>18</superscript> F-FMISO was significantly correlated (all P < 0.05) with TGR, whereas the imaging of <superscript>18</superscript> F-FDG and <superscript>18</superscript> F-ML-10 was not significantly correlated with TGR. Conclusion : Based on the tumor uptake of the PET probes and their correlations with MVD and TGR, <superscript>18</superscript> F-Alfatide II PET may not only monitor the early response but also precisely predict the therapeutic efficacy of the multi-targeted, anti-angiogenic drug sunitinib in U87MG tumors. In conclusion, it is feasible to optimize the early response monitoring and efficacy prediction of cancer AAT using noninvasive PET molecular imaging strategies of multifactorial bioparameters, such as angiogenesis imaging with <superscript>18</superscript> F-Alfatide II, which represents an RGD-based probe.<br />Competing Interests: The authors have declared that no competing interest exists.
- Subjects :
- Animals
Disease Models, Animal
Glioblastoma drug therapy
Glioblastoma pathology
Heterografts
Longitudinal Studies
Mice
Neovascularization, Pathologic drug therapy
Neovascularization, Pathologic pathology
Prognosis
Sunitinib
Angiogenesis Inhibitors administration & dosage
Drug Monitoring methods
Glioblastoma diagnostic imaging
Indoles administration & dosage
Molecular Imaging methods
Neovascularization, Pathologic diagnostic imaging
Positron-Emission Tomography methods
Pyrroles administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1838-7640
- Volume :
- 6
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Theranostics
- Publication Type :
- Academic Journal
- Accession number :
- 27698942
- Full Text :
- https://doi.org/10.7150/thno.13917