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ddpcr: an R package and web application for analysis of droplet digital PCR data.

Authors :
Attali D
Bidshahri R
Haynes C
Bryan J
Source :
F1000Research [F1000Res] 2016 Jun 17; Vol. 5, pp. 1411. Date of Electronic Publication: 2016 Jun 17 (Print Publication: 2016).
Publication Year :
2016

Abstract

Droplet digital polymerase chain reaction (ddPCR) is a novel platform for exact quantification of DNA which holds great promise in clinical diagnostics. It is increasingly popular due to its digital nature, which provides more accurate quantification and higher sensitivity than traditional real-time PCR. However, clinical adoption has been slowed in part by the lack of software tools available for analyzing ddPCR data. Here, we present ddpcr - a new R package for ddPCR visualization and analysis. In addition, ddpcr includes a web application (powered by the Shiny R package) that allows users to analyze ddPCR data using an interactive graphical interface.<br />Competing Interests: No competing interests were disclosed.

Details

Language :
English
ISSN :
2046-1402
Volume :
5
Database :
MEDLINE
Journal :
F1000Research
Publication Type :
Academic Journal
Accession number :
27703666
Full Text :
https://doi.org/10.12688/f1000research.9022.1