Back to Search
Start Over
Molecular beacons with JOE dye: Influence of linker and 3' couple quencher.
- Source :
-
Molecular and cellular probes [Mol Cell Probes] 2016 Oct; Vol. 30 (5), pp. 285-290. Date of Electronic Publication: 2016 Oct 06. - Publication Year :
- 2016
-
Abstract
- Molecular beacons carrying JOE dye (4',5'-dichloro-2',7'-dimethoxy-6-carboxyfluorescein) on a rigid or flexible linker and one or two BHQ1 quenchers have been prepared and tested in real-time PCR using Fusarium avenaceum elongation factor 1α DNA template. The probes were different in their structures (loop size and stem length), linkers for dye attachment (6-aminohexanol or trans-4-aminocyclohexanol), quencher composition (single and double BHQ1) to elucidate the influence of all these features. Fluorogenic properties of the probes were studied and compared to those of FAM (fluorescein)-based probes. All the factors - stem length, JOE vs FAM, rigid vs flexible linker, single vs double quencher - appeared to play a considerable role in the probe's fluorescent properties and determine the usability of the probe at two different temperatures of fluorescence detection (55°С and 64°С).<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1096-1194
- Volume :
- 30
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular and cellular probes
- Publication Type :
- Academic Journal
- Accession number :
- 27720907
- Full Text :
- https://doi.org/10.1016/j.mcp.2016.10.003