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MicroRNA-574-5p promotes metastasis of non-small cell lung cancer by targeting PTPRU.
- Source :
-
Scientific reports [Sci Rep] 2016 Oct 20; Vol. 6, pp. 35714. Date of Electronic Publication: 2016 Oct 20. - Publication Year :
- 2016
-
Abstract
- Dysregulation of microRNAs (miRNAs) has been associated with malignant behavior in a variety of cancers. Our previous study demonstrated that miRNA expression profiles are predictors for patients with advanced non-small cell lung cancer (NSCLC). We also showed that miRNAs are involved in small-cell lung cancer metastasis. Here, we used qRT-PCR to re-analyze our previous microarray results using serum samples from 75 patients with NSCLC. Surprisingly, we found that miR-574-5p and miR-874 were overexpressed in patients with metastatic advanced NSCLC but not in patients with non-metastatic advanced NSCLC. Additionally, miR-574-5p expression was correlated between matched serum and tissue samples from 68 patients. However, these 2 miRNAs are not prognostic factors for NSCLC. Transwell and wound-healing assays showed that miR-574-5p promotes the migration and invasion of NSCLC cells. Furthermore, miR-574-5p enhanced the tyrosine phosphorylation of β-catenin by repressing PTPRU expression in vitro. In conclusion, this study explored the expression of miR-574-5p in clinical samples and its molecular mechanisms in the metastasis of advanced NSCLC.
- Subjects :
- Adult
Aged
Cell Movement drug effects
Female
Humans
Male
Middle Aged
Real-Time Polymerase Chain Reaction
Serum chemistry
Carcinoma, Non-Small-Cell Lung physiopathology
Carcinoma, Non-Small-Cell Lung secondary
MicroRNAs metabolism
Neoplasm Metastasis physiopathology
Receptor-Like Protein Tyrosine Phosphatases, Class 2 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27761023
- Full Text :
- https://doi.org/10.1038/srep35714