Back to Search
Start Over
Cellular thermotolerance is inheritable from Holstein cattle cloned with ooplasts of Taiwan native yellow cattle.
- Source :
-
Theriogenology [Theriogenology] 2017 Jan 15; Vol. 88, pp. 244-253. Date of Electronic Publication: 2016 Sep 22. - Publication Year :
- 2017
-
Abstract
- We have previously demonstrated that the somatic cells from cattle cloned with Holstein (H) donor cells and Taiwan native yellow cattle (Y) ooplasm (Yo-Hd) had better thermotolerance than those from cattle cloned with both Holstein donor cells and ooplasm (Ho-Hd). The present study aimed to investigate whether the cellular thermotolerance of these cloned cattle is transmissible to their offspring (Ho-Hd-F1 and Yo-Hd-F1). Thermotolerance of ear fibroblasts derived from these cloned cattle and their offspring were analyzed. Polymorphisms in mitochondrial DNA (mtDNA) D-loop of ear fibroblasts derived from Yo-Hd and Yo-Hd-F1 indicated that the cytoplasm is originated from Bos indicus (Y). After heat shock, the apoptotic rates, B-cell lymphoma 2-associated X protein/B-cell lymphoma 2 ratios, and relative expression levels of cysteine-aspartic proteases (caspases)-3, -8, and -9 of ear fibroblasts with Y-originated cytoplasm (including Y, Yo-Hd, and Yo-Hd-F1) were lower (P < 0.05) than those of ear fibroblasts with H-originated cytoplasm (including H, Ho-Hd, and Ho-Hd-F1). In contrast, the relative level of HSP-70 was higher (P < 0.05) in ear fibroblasts with Y-originated cytoplasm than that of with H-originated cytoplasm. Based on our results, thermotolerance of ear fibroblasts derived from Yo-Hd and Yo-Hd-F1 cattle is better and can be transmitted, at least at the cellular level, to their offspring.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Caspases genetics
Caspases metabolism
Cattle physiology
Cloning, Organism methods
Cytoplasm physiology
Ear
Fibroblasts cytology
Fibroblasts physiology
Gene Expression Regulation, Developmental physiology
Gene Expression Regulation, Enzymologic
Proto-Oncogene Proteins c-bcl-2 genetics
Proto-Oncogene Proteins c-bcl-2 metabolism
bcl-2-Associated X Protein genetics
bcl-2-Associated X Protein metabolism
Cattle genetics
Cloning, Organism veterinary
Hot Temperature
Stress, Physiological physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3231
- Volume :
- 88
- Database :
- MEDLINE
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 27771117
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2016.09.030