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Study on the effects of gradient mechanical pressures on the proliferation, apoptosis, chondrogenesis and hypertrophy of mandibular condylar chondrocytes in vitro.
- Source :
-
Archives of oral biology [Arch Oral Biol] 2017 Jan; Vol. 73, pp. 186-192. Date of Electronic Publication: 2016 Oct 17. - Publication Year :
- 2017
-
Abstract
- Objective: To investigate the effects of gradient mechanical pressure on chondrocyte proliferation, apoptosis, and the expression of markers of chondrogenesis and chondrocyte hypertrophy.<br />Methods: Mandibular condylar chondrocytes from 5 rabbits were cultured in vitro, and pressed with static pressures of 50kPa, 100kPa, 150kPa and 200kPa for 3h, respectively. The chondrocytes cultured without pressure (0kPa) were used as control. Cell proliferation, apoptosis, and the expression of aggrecan (AGG), collagen II (COL2), collagen X (COL10), alkaline phosphatase (ALP) were investigated. Ultrastructures of the pressurized chondrocytes under transmission electron microscopy (TEM) were observed.<br />Results: Chondrocyte proliferation increased at 100kPa and decreased at 200kPa. Chondrocyte apoptosis increased with peak pressure at 200kPa in a dose-dependent manner. Chondrocyte necrosis increased at 200kPa. The expression of AGG increased at 200kPa. The expression of COL2 decreased at 50kPa and increased at 150kPa. The expression of COL10 and ALP increased at 150kPa. Ultrastructure of the pressurized chondrocytes under TEM showed: at 100kPa, cells were enlarged with less cellular microvillus and a bigger nucleus; at 200kPa, cells shrank with the sign of apoptosis, and apoptosis cells were found.<br />Conclusions: The mechanical loading of 150kPa is the moderate pressure for chondrocyte: cell proliferation and apoptosis is balanced, necrosis is reduced, and chondrogenesis and chondrocyte hypertrophy are promoted. When the pressure is lower, chondrogenesis and chondrocyte hypertrophy are inhibited. At 200kPa, degeneration of cartilage is implied.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Aggrecans biosynthesis
Aggrecans metabolism
Alkaline Phosphatase biosynthesis
Alkaline Phosphatase metabolism
Animals
Apoptosis physiology
Cell Differentiation physiology
Cell Enlargement
Cell Proliferation physiology
Chondrocytes cytology
Chondrocytes metabolism
Collagen biosynthesis
Collagen metabolism
Hypertrophy etiology
Hypertrophy pathology
Mandibular Condyle cytology
Mandibular Condyle metabolism
Mandibular Condyle pathology
Necrosis
Pressure
Rabbits
Chondrocytes physiology
Chondrogenesis physiology
Mandibular Condyle physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1506
- Volume :
- 73
- Database :
- MEDLINE
- Journal :
- Archives of oral biology
- Publication Type :
- Academic Journal
- Accession number :
- 27771587
- Full Text :
- https://doi.org/10.1016/j.archoralbio.2016.10.017