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Pericyte Seeded Dual Peptide Scaffold with Improved Endothelialization for Vascular Graft Tissue Engineering.
- Source :
-
Advanced healthcare materials [Adv Healthc Mater] 2016 Dec; Vol. 5 (23), pp. 3046-3055. Date of Electronic Publication: 2016 Oct 26. - Publication Year :
- 2016
-
Abstract
- The development of synthetic vascular grafts for coronary artery bypass is challenged by insufficient endothelialization, which increases the risk of thrombosis, and the lack of native cellular constituents, which favors pathological remodeling. Here, a bifunctional electrospun poly(ε-caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the osteopontin-derived peptide (Adh) on the "luminal" side and a heparin-binding peptide (Hep) on the "abluminal" side. Additionally, the "abluminal" side of the scaffold is seeded with saphenous vein-derived pericytes (SVPs) as a source of proangiogenic growth factors. The Adh peptide significantly increases endothelial cell adhesion, while the Hep peptide promotes accumulation of vascular endothelial growth factor secreted by SVPs. SVPs increase endothelial migration both in a transwell assay and a modified scratch assay performed on the PCL scaffold. Seeding of SVPs on the "abluminal"/Hep side of the scaffold further increases endothelial cell density, indicating a combinatory effect of the peptides and pericytes. Finally, SVP-seeded scaffolds are preserved by freezing in a xeno-free medium, maintaining good cell viability and function. In conclusion, this engineered scaffold combines patient-derived pericytes and spatially organized functionalities, which synergistically increase endothelial cell density and growth factor retention.<br /> (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Blood Vessel Prosthesis
Cell Adhesion drug effects
Cell Movement drug effects
Cell Survival drug effects
Cells, Cultured
Coronary Vessels drug effects
Coronary Vessels metabolism
Endothelial Cells metabolism
Heparin metabolism
Humans
Osteopontin metabolism
Peptides chemistry
Pericytes metabolism
Polyesters administration & dosage
Polyesters chemistry
Tissue Engineering methods
Vascular Endothelial Growth Factor A metabolism
Endothelial Cells drug effects
Peptides administration & dosage
Pericytes drug effects
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2192-2659
- Volume :
- 5
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Advanced healthcare materials
- Publication Type :
- Academic Journal
- Accession number :
- 27782370
- Full Text :
- https://doi.org/10.1002/adhm.201600699