Back to Search
Start Over
Fine Mapping of a New Race-Specific Blast Resistance Gene, Pi-hk2, in Japonica Heikezijing from Taihu Region of China.
- Source :
-
Phytopathology [Phytopathology] 2017 Jan; Vol. 107 (1), pp. 84-91. Date of Electronic Publication: 2016 Nov 07. - Publication Year :
- 2017
-
Abstract
- Heikezijing, a japonica rice landrace from the Taihu region of China, exhibited broad-spectrum resistance to more than 300 isolates of the blast pathogen (Magnaporthe oryzae). In our previous research, we fine mapped a broad-spectrum resistance gene, Pi-hk1, in chromosome 11. In this research, 2010-9(G1), one of the predominant races of blast in the Taihu Lake region of China, was inoculated into 162 recombinant inbred lines (RIL) and two parents, Heikezijing and Suyunuo, for mapping the resistance-blast quantitative trait loci (QTL). Three QTL (Lsqtl4-1, Lsqtl9-1, and Lsqtl11-1) associated with lesion scores were detected on chromosomes 4, 9, and 11 and two QTL (Lnqtl1-1 and Lnqtl9-1) associated with average lesion numbers were detected on chromosomes 1 and 9. The QTL Lsqtl9-1 conferring race-specific resistance to 2010-9(G1) at seedling stages showed logarithm of the odds scores of 9.10 and phenotypic variance of 46.19% and might be a major QTL, named Pi-hk2. The line RIL84 with Pi-hk2 derived from a cross between Heikezijing and Suyunuo was selected as Pi-hk2 gene donor for developing fine mapping populations. According to the resistance evaluation of recombinants of three generations (BC <subscript>1</subscript> F <subscript>2</subscript> , BC <subscript>1</subscript> F <subscript>3</subscript> , and BC <subscript>1</subscript> F <subscript>4</subscript> ), Pi-hk2 was finally mapped to a 143-kb region between ILP-19 and RM24048, and 18 candidate genes were predicted, including genes that encode pleiotropic drug resistance protein 4 (n = 2), WRKY74 (n = 1), cytochrome b5-like heme/steroid-binding domain containing protein (n = 1), protein kinase (n = 1), and ankyrin repeat family protein (n = 1). These results provide essential information for cloning of Pi-hk2 and its potential utility in breeding resistant rice cultivars by marker-assisted selection.
- Subjects :
- Breeding
China
Chromosome Mapping
Gene Expression Profiling
Genetic Markers genetics
Oligonucleotide Array Sequence Analysis
Organ Specificity
Oryza immunology
Oryza microbiology
Phenotype
Plant Diseases microbiology
Plant Leaves genetics
Plant Leaves immunology
Plant Leaves microbiology
Plant Proteins metabolism
Seedlings genetics
Seedlings immunology
Seedlings microbiology
Disease Resistance genetics
Magnaporthe physiology
Oryza genetics
Plant Diseases immunology
Plant Proteins genetics
Quantitative Trait Loci genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0031-949X
- Volume :
- 107
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Phytopathology
- Publication Type :
- Academic Journal
- Accession number :
- 27819540
- Full Text :
- https://doi.org/10.1094/PHYTO-03-16-0151-R