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Microencapsulation of Hepatocytes and Mesenchymal Stem Cells for Therapeutic Applications.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1506, pp. 259-271. - Publication Year :
- 2017
-
Abstract
- Encapsulated hepatocyte transplantation and encapsulated mesenchymal stem cell transplantation are newly developed potential treatments for acute and chronic liver diseases, respectively. Cells are microencapsulated in biocompatible semipermeable alginate-based hydrogels. Microspheres protect cells against antibodies and immune cells, while allowing nutrients, small/medium size proteins and drugs to diffuse inside and outside the polymer matrix. Microencapsulated cells are assessed in vitro and designed for experimental transplantation and for future clinical applications.Here, we describe the protocol for microencapsulation of hepatocytes and mesenchymal stem cells within hybrid poly(ethylene glycol)-alginate hydrogels.
- Subjects :
- Alginates chemistry
Animals
Biocompatible Materials chemistry
Cell Differentiation
Cell Proliferation
Cell Survival
Cells, Cultured
Disease Models, Animal
Drug Compounding instrumentation
Glucuronic Acid chemistry
Hepatocytes physiology
Hexuronic Acids chemistry
Humans
Hydrogels chemistry
Immunochemistry
Liver cytology
Liver pathology
Mesenchymal Stem Cell Transplantation instrumentation
Mesenchymal Stem Cell Transplantation mortality
Mesenchymal Stem Cells physiology
Mice
Polyethylene Glycols chemistry
Primary Cell Culture methods
Survival Analysis
Capsules chemistry
Drug Compounding methods
End Stage Liver Disease therapy
Hepatocytes transplantation
Mesenchymal Stem Cell Transplantation methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1506
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 27830559
- Full Text :
- https://doi.org/10.1007/978-1-4939-6506-9_18