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A "turn-off" fluorescent biosensor for the detection of mercury (II) based on graphite carbon nitride.
- Source :
-
Talanta [Talanta] 2017 Jan 01; Vol. 162, pp. 46-51. Date of Electronic Publication: 2016 Oct 04. - Publication Year :
- 2017
-
Abstract
- A novel and simple fluorescent biosensor has been realized through the fluorescence quenching of graphite carbon nitride (g-C <subscript>3</subscript> N <subscript>4</subscript> ) for mercuric ion (Hg <superscript>2+</superscript> ) detection. In this assay, the g-C <subscript>3</subscript> N <subscript>4</subscript> sheets which were functionalized with single-stranded DNA (ssDNA) aptamer showed strong fluorescence emission at 440nm under the excitation of 380nm in the absence of Hg <superscript>2+</superscript> . When added to the assay solution, Hg <superscript>2+</superscript> was embedded in the hairpin-shaped double-stranded DNA (dsDNA) due to the formation of the thymine-Hg <superscript>2+</superscript> -thymine (T-Hg <superscript>2+</superscript> -T) complex, which made the Hg <superscript>2+</superscript> close to the surface of g-C <subscript>3</subscript> N <subscript>4</subscript> sheet. Therefore, the fluorescence of g-C <subscript>3</subscript> N <subscript>4</subscript> was quenched. This sensor has good selectivity with a limit of detection as low as 0.17nM under optimal conditions. The present work demonstrates that the g-C <subscript>3</subscript> N <subscript>4</subscript> -based fluorescent sensor has a promising application for detection of metal ions in real samples.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 162
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 27837856
- Full Text :
- https://doi.org/10.1016/j.talanta.2016.09.066