Quick and cheap MIRU-VNTR typing of Mycobacterium tuberculosis species complex using duplex PCR.

Authors :: Yasmin M
Le Moullec S
Siddiqui RT
De Beer J
Sola C
Refrégier G
Source :: Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2016 Dec; Vol. 101, pp. 160-163. Date of Electronic Publication: 2016 Oct 03.
Publication Year :: 2016
Abstract: While minisatellites are usually typed using capillary sequencers or qiaplex systems in developed countries, many low-resource regions cannot afford it. We propose an optimized agarose gel electrophoresis method to genotype Mycobacterium tuberculosis species complex minisatellites in their standardized format (24 MIRU-VNTR). It is based on duplex PCRs combining VNTR loci harboring distinct amplicon sizes whatever the repetition number of each locus. This method performs well both on DNA extracts of good quality and on thermolysates while reducing workload and reagents costs.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Subjects :: DNA, Bacterial genetics
Electrophoresis, Agar Gel methods
Genotyping Techniques methods
Humans
Minisatellite Repeats genetics
Multiplex Polymerase Chain Reaction methods
Tuberculosis microbiology
Bacterial Typing Techniques methods
Mycobacterium tuberculosis classification
Mycobacterium tuberculosis genetics

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