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[Extraction and identification of semen-derived exosomes using PEG6000].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2016 Nov 20; Vol. 36 (11), pp. 1531-1535. - Publication Year :
- 2016
-
Abstract
- Objective: To extract and identify semen-derived exosome using PEG6000.<br />Methods: Exosomes were extracted from semen specimens from 6 healthy volunteers with step-by-step centrifugations and ultracentrifugation prior to 8% PEG6000 enrichment. The extracted exosomes were characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting.<br />Results: The pellets obtained were round or elliptic membrane vesicles 30 to 150 nm in diameter with intact double membranes and contained low electron density material. The pellets expressed CD63, ALIX and TSG101 molecules but not calnexin that was expressed in sperm cells.<br />Conclusion: The PEG6000-based method for extraction of exosomes from semen samples facilitates future studies of seminal exosomes.
- Subjects :
- Blotting, Western
Calcium-Binding Proteins metabolism
Cell Cycle Proteins metabolism
Centrifugation
DNA-Binding Proteins metabolism
Endosomal Sorting Complexes Required for Transport metabolism
Humans
Male
Microscopy, Electron, Transmission
Nanoparticles
Polyethylene Glycols
Spermatozoa
Tetraspanin 30 metabolism
Transcription Factors metabolism
Ultracentrifugation
Exosomes chemistry
Semen chemistry
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 36
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 27881345