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Different routes and doses influence protection in pigs immunised with the naturally attenuated African swine fever virus isolate OURT88/3.
- Source :
-
Antiviral research [Antiviral Res] 2017 Feb; Vol. 138, pp. 1-8. Date of Electronic Publication: 2016 Nov 28. - Publication Year :
- 2017
-
Abstract
- This study compares different combinations of doses and routes of immunisation of pigs with low virulent African swine fever virus (ASFV) genotype I isolate OURT88/3, including the intramuscular and intranasal route, the latter not previously tested. Intranasal immunisations with low and moderate doses (10 <superscript>3</superscript> and 10 <superscript>4</superscript> TCID <subscript>50</subscript> ) of OURT88/3 provided complete protection (100%) against challenge with virulent genotype I OURT88/1 isolate. Only mild and transient clinical reactions were observed in protected pigs. Transient moderate virus genome levels were detected in blood samples after challenge that decreased, but persisted until the end of the experiment in some animals. In contrast, pigs immunised intramuscularly with low and moderate doses (10 <superscript>3</superscript> and 10 <superscript>4</superscript> TCID <subscript>50</subscript> ) displayed lower percentages of protection (50-66%), and low or undetectable levels of virus genome were detected in blood samples throughout the study. In addition, clinical courses observed in protected pigs were asymptomatic. In pigs that were not protected and developed acute ASF, an exacerbated increase of IL-10 sometimes accompanied by an increase of IFNγ was observed before euthanasia. These results showed that factors including delivery route and dose determine the outcome of immunisation with the naturally attenuated isolate OURT88/3.<br /> (Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Administration, Intranasal
African Swine Fever virology
African Swine Fever Virus genetics
African Swine Fever Virus isolation & purification
African Swine Fever Virus pathogenicity
Animals
Antibodies, Viral blood
DNA, Viral blood
Genome, Viral
Injections, Intramuscular
Interferon-gamma genetics
Interleukin-10 genetics
Swine
Vaccination methods
Vaccines, Attenuated administration & dosage
Viral Load
African Swine Fever prevention & control
African Swine Fever Virus immunology
Vaccination veterinary
Viral Vaccines administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9096
- Volume :
- 138
- Database :
- MEDLINE
- Journal :
- Antiviral research
- Publication Type :
- Academic Journal
- Accession number :
- 27908827
- Full Text :
- https://doi.org/10.1016/j.antiviral.2016.11.021