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Diverse Mesorhizobium bacteria nodulate native Astragalus and Oxytropis in arctic and subarctic areas in Eurasia.
- Source :
-
Systematic and applied microbiology [Syst Appl Microbiol] 2017 Jan; Vol. 40 (1), pp. 51-58. Date of Electronic Publication: 2016 Nov 30. - Publication Year :
- 2017
-
Abstract
- Rhizobia nodulating native Astragalus and Oxytropis spp. in Northern Europe are not well-studied. In this study, we isolated bacteria from nodules of four Astragalus spp. and two Oxytropis spp. from the arctic and subarctic regions of Sweden and Russia. The phylogenetic analyses were performed by using sequences of three housekeeping genes (16S rRNA, rpoB and recA) and two accessory genes (nodC and nifH). The results of our multilocus sequence analysis (MLSA) of the three housekeeping genes tree showed that all the 13 isolates belonged to the genus Mesorhizobium and were positioned in six clades. Our concatenated housekeeping gene tree also suggested that the isolates nodulating Astragalus inopinatus, Astragalus frigidus, Astragalus alpinus ssp. alpinus and Oxytropis revoluta might be designated as four new Mesorhizobium species. The 13 isolates were grouped in three clades in the nodC and nifH trees. <superscript>15</superscript> N analysis suggested that the legumes in association with these isolates were actively fixing nitrogen.<br /> (Copyright © 2016 Elsevier GmbH. All rights reserved.)
- Subjects :
- Arctic Regions
Bacterial Proteins genetics
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
DNA-Directed RNA Polymerases genetics
Mesorhizobium genetics
Multilocus Sequence Typing
N-Acetylglucosaminyltransferases genetics
Oxidoreductases genetics
Phylogeny
RNA, Ribosomal, 16S genetics
Rec A Recombinases genetics
Russia
Sweden
Astragalus Plant microbiology
Genetic Variation
Mesorhizobium classification
Mesorhizobium isolation & purification
Oxytropis microbiology
Plant Root Nodulation
Root Nodules, Plant microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0984
- Volume :
- 40
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Systematic and applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 27939530
- Full Text :
- https://doi.org/10.1016/j.syapm.2016.11.004