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Molecular evidence on the protective effect of ellagic acid on phosalone-induced senescence in rat embryonic fibroblast cells.
- Source :
-
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2017 Feb; Vol. 100, pp. 8-23. Date of Electronic Publication: 2016 Dec 11. - Publication Year :
- 2017
-
Abstract
- Salient evidence testifies the link between organophosphorus (OPs) exposure and the formation of free radical oxidants; and it is well accepted that free radicals are one of the basic concerns of senescence. To show the oxidative features of phosalone (PLN) as a key member of OPs, to induce senescence in rat embryonic fibroblast (REF) cells and to demonstrate the beneficial effects of the known antioxidant ellagic acid (EA) in diminishing the PLN-induced toxic effects, the levels of cell viability, oxidative stress markers, inflammatory cytokines, telomerase activity, and the expression of the genes related to senescence were investigated. Our results lend support to the hypothesis that PLN enhances the entire premature senescence parameters of REF cells. This accounts for the mechanistic approval of the role of OPs in induction of senescence in rat fibroblasts. Moreover, incorporation of EA diminished PLN toxicity mainly through suppression of p38 and p53 at gene and protein levels, and tempered the inflammation factors (TNF-α, IL-1β, IL-6 and NF-κB), which further affected cell division. Analysis of cell cycle showed that the percentage of G0/G1 arrest, in REF cells treated by EA was elevated as compared to control and PLN treated cells.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antioxidants metabolism
Cell Cycle drug effects
Cell Survival drug effects
Cells, Cultured
Embryo, Mammalian pathology
Female
Fibroblasts pathology
Insecticides toxicity
Pregnancy
Rats
Rats, Wistar
Cellular Senescence drug effects
Ellagic Acid pharmacology
Embryo, Mammalian drug effects
Fibroblasts drug effects
Organothiophosphorus Compounds toxicity
Oxidative Stress drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6351
- Volume :
- 100
- Database :
- MEDLINE
- Journal :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- Publication Type :
- Academic Journal
- Accession number :
- 27965107
- Full Text :
- https://doi.org/10.1016/j.fct.2016.12.008