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Specific targeting delivery to MUC1 overexpressing tumors by albumin-chitosan nanoparticles conjugated to DNA aptamer.
- Source :
-
International journal of pharmaceutics [Int J Pharm] 2016 Dec 30; Vol. 515 (1-2), pp. 607-615. Date of Electronic Publication: 2016 Oct 29. - Publication Year :
- 2016
-
Abstract
- Chitosan-coated human serum albumin nanoparticles were functionalized by MUC1 aptamer to obtain a selective drug carrier toward cancers overexpressing MUC1. The negative charges of albumin nanoparticles were shifted to positive charges by surface modification with chitosan, and MUC1 was conjugated through an acrylate spacer. The cytotoxicity of targeted nanoparticles was significantly more than non-aptamer nanoparticles, and also the chitosan-coated nanoparticles had more cytotoxic effects than the negatively charged albumin nanoparticles. The IC <subscript>50</subscript> of targeted nanoparticles was 28 and 26% of free paclitaxel in MCF7 and T47D cells at 48h, respectively. Confocal laser scanning electron microscopy showed that aptamer conjugation and positive charge increase the cellular uptake. 66% of paclitaxel was released within 32h, but 100% of drug was released at pH=5.5 (similar cancer cells). The paclitaxel plasma amount was at a good level of 17.6% at 2h for increasing the chance of cellular uptake.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Aptamers, Nucleotide chemistry
Aptamers, Nucleotide genetics
Cell Line, Tumor
Chitosan administration & dosage
Chitosan chemistry
Chitosan pharmacokinetics
Drug Carriers administration & dosage
Drug Carriers pharmacokinetics
Female
Humans
MCF-7 Cells
Molecular Targeted Therapy
Mucin-1 genetics
Mucin-1 metabolism
Nanoparticles chemistry
Nanoparticles metabolism
Paclitaxel pharmacology
Serum Albumin administration & dosage
Serum Albumin chemistry
Serum Albumin pharmacokinetics
Aptamers, Nucleotide administration & dosage
Breast Neoplasms drug therapy
Breast Neoplasms metabolism
Mucin-1 biosynthesis
Nanoparticles administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3476
- Volume :
- 515
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- International journal of pharmaceutics
- Publication Type :
- Academic Journal
- Accession number :
- 27989825
- Full Text :
- https://doi.org/10.1016/j.ijpharm.2016.10.066