Back to Search
Start Over
Chitosan-Coated Diatom Silica as Hemostatic Agent for Hemorrhage Control.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2016 Dec 21; Vol. 8 (50), pp. 34234-34243. Date of Electronic Publication: 2016 Dec 08. - Publication Year :
- 2016
-
Abstract
- Uncontrolled hemorrhage leads to high death risk both in military and civilian trauma. Current hemostatic agents still have various limitations and side effects. In this study, natural diatom silica obtained from diatomite and diatom culture was purified and developed for hemorrhage control. To improve the biocompatibility and hemostatic performance of diatom silica, a series of chitosan-coated diatom (CS-diatom) was developed. The composition of CS-diatom prepared was optimized by in vitro hemocompatibility and blood coagulation evaluation for that prepared with 0.5%, 1%, 3%, and 5% chitosan. The results demonstrated that the CS-diatom prepared with 1% chitosan exhibited favorable biocompatibility (hemolysis ratio < 5%, no cytotoxicity to MEFs), great fluid absorbility (24.39 ± 1.53 times the weight of liquid), and desirable hemostasis effect (351 ± 14.73 s at 5 mg/mL, 248 ± 32.42s at 10 mg/mL). Further blood coagulation mechanism study indicated that CS-diatom could provide an ideal interface to induce erythrocyte absorption and aggregation, along with activating the intrinsic coagulation pathway and thus accelerated blood coagulation. Benefitting from the multiple hemostatic performances, CS-diatom showed the shortest clotting time (98.34 ± 26.54 s) and lowest blood loss (0.31 ± 0.11 g) in rat-tail amputation model compare to diatomite and diatom as well as gauze and commercial QuikClot zeolite. The results evidenced that the CS-diatom was a safe and effective hemostatic agent and provided a new understanding of nonsynthetic mesoporous materials for hemorrhage control.
- Subjects :
- Animals
Blood Coagulation drug effects
Cell Line
Chitosan administration & dosage
Chitosan adverse effects
Diatoms chemistry
Fibroblasts drug effects
Hemostasis drug effects
Hemostatics adverse effects
Mice
Rabbits
Rats
Rats, Sprague-Dawley
Silicon Dioxide administration & dosage
Silicon Dioxide adverse effects
Chitosan chemistry
Drug Delivery Systems methods
Hemorrhage drug therapy
Hemostatics administration & dosage
Hemostatics chemistry
Silicon Dioxide chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 8
- Issue :
- 50
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 27998147
- Full Text :
- https://doi.org/10.1021/acsami.6b12317