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A single dose of epidermicin NI01 is sufficient to eradicate MRSA from the nares of cotton rats.
- Source :
-
The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2017 Mar 01; Vol. 72 (3), pp. 778-781. - Publication Year :
- 2017
-
Abstract
- Objectives: To investigate the efficacy of a potent novel antimicrobial protein of mass 6 kDa, epidermicin NI01, for eradicating the nasal burden of MRSA in a cotton rat ( Sigmodon hispidus ) model.<br />Methods: MRSA strain ATCC 43300 was used to establish a robust colonization of cotton rat nares. This model was used to evaluate the efficacy of topical 0.04% and 0.2% epidermicin NI01, administered twice daily for 3 days consecutively, and topical 0.8% epidermicin NI01 administered once, for reducing nasal MRSA burden. Control groups remained untreated or were administered vehicle only (0.5% hydroxypropylmethylcellulose) or 2% mupirocin twice daily for 3 days. The experiment was terminated at day 5 and MRSA quantitative counts were determined. Tissues recovered from animals treated with 0.2% epidermicin twice daily for 3 days were examined for histological changes.<br />Results: Mupirocin treatment resulted in a reduction in burden of log 10 (log R) of 2.59 cfu/nares compared with vehicle ( P < 0.0001). Epidermicin NI01 administered once at 0.8% showed excellent efficacy, resulting in a log R of 2.10 cfu/nares ( P = 0.0004), which was equivalent to mupirocin. Epidermicin NI01 administered at 0.2% or 0.04% twice daily for 3 days did not have a significant impact on the tissue burden recovered from the nares. Mild to marked histological abnormalities were noted, but these were determined to be reversible.<br />Conclusion: A single dose of topical epidermicin NI01 was as effective as mupirocin administered twice daily for 3 days in eradication of MRSA from the nares of cotton rats. This justifies further development of epidermicin for this indication.<br /> (© The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.)
- Subjects :
- Administration, Topical
Animals
Antimicrobial Cationic Peptides therapeutic use
Bacterial Load drug effects
Bacteriocins therapeutic use
Dose-Response Relationship, Drug
Mupirocin therapeutic use
Nose drug effects
Rats
Sigmodontinae
Staphylococcal Infections microbiology
Antimicrobial Cationic Peptides administration & dosage
Bacteriocins administration & dosage
Methicillin-Resistant Staphylococcus aureus drug effects
Nose microbiology
Staphylococcal Infections drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2091
- Volume :
- 72
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of antimicrobial chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 27999015
- Full Text :
- https://doi.org/10.1093/jac/dkw457