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Evaluation of OMNIgene®•SPUTUM-stabilised sputum for long-term transport and Xpert® MTB/RIF testing in Nepal.
- Source :
-
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease [Int J Tuberc Lung Dis] 2016 Dec 01; Vol. 20 (12), pp. 1661-1667. - Publication Year :
- 2016
-
Abstract
- Setting: German Nepal TB Project, National Tuberculosis Reference Laboratory, Kathmandu, Nepal.<br />Objective: To evaluate whether transporting samples in OMNIgene®•SPUTUM (OM-S) reagent from a peripheral collection site to a central laboratory in Nepal can improve tuberculosis (TB) detection and increase the sensitivity of Xpert® MTB/RIF testing.<br />Design: One hundred sputum samples were split manually. Each portion was assigned to the OM-S group (OM-S added at collection, airline-couriered without cold chain, no other processing required) or the standard-of-care (SOC) group (samples airline-couriered on ice, sodium hydroxide + N-acetyl-L-cysteine processing required at the laboratory). Smear microscopy and Xpert testing were performed.<br />Results: Transport time was 2-13 days. Overall smear results were comparable (respectively 58% and 56% smear-negative results in the OM-S and SOC groups). The rate of smear-positive, Mycobacterium tuberculosis-positive (MTB+) sample detection was identical for both treatment groups, at 95%. More smear-negative MTB+ samples were detected in the OM-S group (17% vs. 13%, P = 0.0655).<br />Conclusion: Sputum samples treated with OM-S can undergo multiday ambient-temperature transport and yield comparable smear and Xpert results to those of SOC samples. Further investigation with larger sample sizes is required to assess whether treating sputum samples with OM-S could increase the sensitivity of Xpert testing in smear-negative samples.
Details
- Language :
- English
- ISSN :
- 1815-7920
- Volume :
- 20
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
- Publication Type :
- Academic Journal
- Accession number :
- 28000586
- Full Text :
- https://doi.org/10.5588/ijtld.16.0421