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ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2016 Dec 21; Vol. 22 (47), pp. 10364-10370. - Publication Year :
- 2016
-
Abstract
- Aim: To explore expression of angiopoietin-like protein 2 (ANGPTL2) and its effect on biological behavior such as proliferation and invasiveness in gastric cancer.<br />Methods: Western blotting was used to detect expression of ANGPTL2 in 60 human normal gastric tissues, 60 human gastric cancer tissues and gastric cell lines including GES-1, N87, SGC7901, BGC823 and PAMC82. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assay were used to detect the proliferation and invasive ability of gastric cancer cells.<br />Results: Compared to normal tissues, ANGPTL2 protein levels were significantly upregulated in gastric tissues, and this level was closely correlated with gastric tumor grade, clinical stage and lymph node metastasis. Compared to GES-1 cells, ANGPTL2 mRNA and protein levels were significantly increased in gastric cancer cells including N87, SGC7901, BGC823 and PAMC82. The expression of ANGPTL2 in highly malignant gastric cancer cell lines BGC823 and PAMC82 was significantly higher than in low malignancy gastric cancer cell lines N87 and SGC7901. MTT and Transwell experiments indicated that the proliferation rate and invasive ability of stable overexpressed gastric cancer cells was faster than in cells transfected with Lv-NC and blank control cells, and the invasive ability of stable overexpressed gastric cancer cells was higher than that of cells transfected with Lv-NC and blank control cells.<br />Conclusion: ANGPTL2 contributed to proliferation and invasion of gastric cancer cells. In clinical treatment, ANGPTL2 may become a new target for treatment of gastric cancer.<br />Competing Interests: Conflict-of-interest statement: We declare that there are no conflicts of interest to disclose.
- Subjects :
- Adult
Aged
Aged, 80 and over
Angiopoietin-Like Protein 2
Angiopoietin-like Proteins
Angiopoietins genetics
Cell Differentiation
Cell Line, Tumor
Female
Humans
Lymphatic Metastasis
Male
Middle Aged
Neoplasm Grading
Neoplasm Invasiveness
Neoplasm Staging
RNA Interference
Signal Transduction
Stomach Neoplasms genetics
Stomach Neoplasms pathology
Transfection
Up-Regulation
Angiopoietins metabolism
Cell Movement
Cell Proliferation
Stomach Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 22
- Issue :
- 47
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 28058016
- Full Text :
- https://doi.org/10.3748/wjg.v22.i47.10364