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Role of REM Sleep, Melanin Concentrating Hormone and Orexin/Hypocretin Systems in the Sleep Deprivation Pre-Ischemia.
- Source :
-
PloS one [PLoS One] 2017 Jan 06; Vol. 12 (1), pp. e0168430. Date of Electronic Publication: 2017 Jan 06 (Print Publication: 2017). - Publication Year :
- 2017
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Abstract
- Study Objectives: Sleep reduction after stroke is linked to poor recovery in patients. Conversely, a neuroprotective effect is observed in animals subjected to acute sleep deprivation (SD) before ischemia. This neuroprotection is associated with an increase of the sleep, melanin concentrating hormone (MCH) and orexin/hypocretin (OX) systems. This study aims to 1) assess the relationship between sleep and recovery; 2) test the association between MCH and OX systems with the pathological mechanisms of stroke.<br />Methods: Sprague-Dawley rats were assigned to four experimental groups: (i) SD&#95;IS: SD performed before ischemia; (ii) IS: ischemia; (iii) SD&#95;Sham: SD performed before sham surgery; (iv) Sham: sham surgery. EEG and EMG were recorded. The time-course of the MCH and OX gene expression was measured at 4, 12, 24 hours and 3, 4, 7 days following ischemic surgery by qRT-PCR.<br />Results: A reduction of infarct volume was observed in the SD&#95;IS group, which correlated with an increase of REM sleep observed during the acute phase of stroke. Conversely, the IS group showed a reduction of REM sleep. Furthermore, ischemia induces an increase of MCH and OX systems during the acute phase of stroke, although, both systems were still increased for a long period of time only in the SD&#95;IS group.<br />Conclusions: Our data indicates that REM sleep may be involved in the neuroprotective effect of SD pre-ischemia, and that both MCH and OX systems were increased during the acute phase of stroke. Future studies should assess the role of REM sleep as a prognostic marker, and test MCH and OXA agonists as new treatment options in the acute phase of stroke.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Animals
Electroencephalography
Electromyography
Gene Expression
Hypothalamic Hormones genetics
Male
Melanins genetics
Orexins genetics
Pituitary Hormones genetics
Prognosis
Rats, Sprague-Dawley
Stroke genetics
Hypothalamic Hormones physiology
Melanins physiology
Orexins physiology
Pituitary Hormones physiology
Sleep Deprivation
Sleep, REM
Stroke physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 28061506
- Full Text :
- https://doi.org/10.1371/journal.pone.0168430