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Antimicrobial activity of photodynamic therapy in combination with colistin against a pan-drug resistant Acinetobacter baumannii isolated from burn patient.
- Source :
-
Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2017 Jun; Vol. 18, pp. 1-5. Date of Electronic Publication: 2017 Jan 12. - Publication Year :
- 2017
-
Abstract
- Nosocomially-acquired multi-, extensively-, and pandrug resistant (MDR, XDR, and PDR) strains of microorganisms such as Acinetobacter baumannii remain a serious cause of infection and septic mortality in burn patients. Treatment of patients with nosocomial burn wound infections is often complicated by drug-resistant strains of A. baumannii. Today, many researchers are focusing on the investigation of novel non-antibiotic strategies such as photodynamic therapy (PDT). We report a new PDT strategy that suppresses colistin resistance in PDR A. baumannii by interfering with the expression of a pmrA/pmrB two-component system. In the current study, A. baumannii with a PDR feature isolated from a burn patient was used as a test strain. PDT was carried out using toluidine blue O (TBO) and light-emitting diode (LED) as a photosensitizer and radiation source, respectively. The antimicrobial susceptibility profiles were assessed for cells surviving PDT. The effects of sub-lethal PDT (sPDT) on the expression of the pmrA/pmrB two-component signal transduction system were evaluated by real-time quantitative reverse transcription PCR. Results of drug susceptibly testing (DST) in LED and TBO groups separately showed that the bacteria were resistant to all tested antibiotics, while the DST result of the LED+TBO group showed highly declining bacterial growth when compared with the control group. Reduction in the expression of pmrA and pmrB was observed in the treated strains after sPDT. This represents the first conclusive example of a direct role for the PDT in breaking antibiotic resistance by directly modulating two-component system activity.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Acinetobacter Infections microbiology
Acinetobacter Infections pathology
Acinetobacter baumannii radiation effects
Anti-Bacterial Agents administration & dosage
Anti-Bacterial Agents pharmacology
Burns drug therapy
Burns microbiology
Combined Modality Therapy
Decontamination
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Drug Resistance, Multiple, Bacterial radiation effects
Humans
Photosensitizing Agents administration & dosage
Photosensitizing Agents pharmacology
Tolonium Chloride administration & dosage
Tolonium Chloride pharmacology
Treatment Outcome
Acinetobacter Infections drug therapy
Acinetobacter baumannii drug effects
Colistin administration & dosage
Colistin pharmacology
Drug Resistance, Multiple, Bacterial drug effects
Photochemotherapy
Subjects
Details
- Language :
- English
- ISSN :
- 1873-1597
- Volume :
- 18
- Database :
- MEDLINE
- Journal :
- Photodiagnosis and photodynamic therapy
- Publication Type :
- Academic Journal
- Accession number :
- 28088439
- Full Text :
- https://doi.org/10.1016/j.pdpdt.2017.01.003