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Involvement of p53 and Bcl-2 in sensory cell degeneration in aging rat cochleae.
- Source :
-
Acta oto-laryngologica [Acta Otolaryngol] 2017 Jun; Vol. 137 (6), pp. 572-580. Date of Electronic Publication: 2017 Jan 17. - Publication Year :
- 2017
-
Abstract
- Conclusion: p53 and Bcl-2 (B-cell lymphoma 2) are involved in the process of sensory cell degeneration in aging cochleae.<br />Objective: To determine molecular players in age-related hair cell degeneration, this study examined the changes in p53 and Bcl-2 expression at different stages of apoptotic and necrotic death of hair cells in aging rat cochleae.<br />Methods: Young (3-4 months) and aging (23-24 months) Fisher 344/NHsd rats were used. The thresholds of the auditory brainstem response (ABR) were measured to determine the auditory function. Immunolabeling was performed to determine the expression of p53 and Bcl-2 proteins in the sensory epithelium. Propidium iodide staining was performed to determine the morphologic changes in hair cell nuclei.<br />Results: Aging rats exhibited a significant elevation in ABR thresholds at all tested frequencies (pā<ā0.001). The p53 and Bcl-2 immunoreactivity was increased in aging hair cells showing the early signs of apoptotic changes in their nuclei. The Bcl-2 expression increase was also observed in hair cells displaying early signs of necrosis. As the hair cell degenerative process advanced, p53 and Bcl-2 immunoreactivity became reduced or absent. In the areas where no detectable nuclear staining was present, p53 and Bcl-2 immunoreactivity was absent.
- Subjects :
- Aging pathology
Animals
Apoptosis
Hair Cells, Auditory pathology
Male
Necrosis
Presbycusis metabolism
Rats, Inbred F344
Aging metabolism
Evoked Potentials, Auditory, Brain Stem
Hair Cells, Auditory metabolism
Presbycusis etiology
Proto-Oncogene Proteins c-bcl-2 metabolism
Tumor Suppressor Protein p53 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1651-2251
- Volume :
- 137
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Acta oto-laryngologica
- Publication Type :
- Academic Journal
- Accession number :
- 28093932
- Full Text :
- https://doi.org/10.1080/00016489.2016.1274425