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Gigantol from Dendrobium chrysotoxum Lindl. binds and inhibits aldose reductase gene to exert its anti-cataract activity: An in vitro mechanistic study.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2017 Feb 23; Vol. 198, pp. 255-261. Date of Electronic Publication: 2017 Jan 16. - Publication Year :
- 2017
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Abstract
- Ethnopharmacological Relevance: Dendrobium. chrysotoxum Lindl is a commonly used species of medicinal Dendrobium which belongs to the family of Orchidaceae, locally known as "Shihu" or "Huangcao". D. chrysotoxum Lindl is widely known for medicinal values in traditional Chinese medicine as it possesses anti-inflammatory, anti-hyperglycemic induction, antitumor and antioxidant properties.<br />Study Aim: To characterize the interaction between gigantol extracted from D. chrysotoxum Lindl and the AR gene, and determine gigantol's efficacy against cataractogenesis.<br />Materials and Methods: Human lens epithelial cells (HLECs) were induced by glucose as the model group. Reverse transcription polymerase chain reaction (RT-PCR) was used to assess AR gene expression. Then, the mode of interaction of gigantol with the AR gene was evaluated by UV-visible spectroscopy, atomic force microscope (AFM) and surface-enhanced Raman spectroscopy (SERS). The binding constant was determined by UV-visible.<br />Results: Gigantol depressed AR gene expression in HLECs. UV-visible spectra preliminarily indicated that interaction between the AR gene and gigantol may follow the groove mode, with a binding constant of 1.85×10 <superscript>3</superscript> L/mol. Atomic force microscope (AFM) data indicated that gigantol possibly bound to insert AR gene base pairs of the double helix. Surface-enhanced Raman spectroscopy (SERS) studies further supported these observations.<br />Conclusion: Gigantol extracted from D. chrysotoxum Lindl not only has inhibitory effects on aldose reductase, but also inhibits AR gene expression. These findings provide a more comprehensive theoretical basis for the use of Dendrobium for the treatment of diabetic cataract.<br /> (Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Bibenzyls isolation & purification
Cataract etiology
Cells, Cultured
Diabetes Complications prevention & control
Epithelial Cells drug effects
Epithelial Cells enzymology
Gene Expression Regulation, Enzymologic drug effects
Glucose metabolism
Guaiacol isolation & purification
Guaiacol pharmacology
Humans
Lens, Crystalline cytology
Lens, Crystalline drug effects
Lens, Crystalline enzymology
Reverse Transcriptase Polymerase Chain Reaction
Spectrum Analysis, Raman
Aldehyde Reductase genetics
Bibenzyls pharmacology
Cataract prevention & control
Dendrobium chemistry
Guaiacol analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 198
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 28104409
- Full Text :
- https://doi.org/10.1016/j.jep.2017.01.026