Back to Search
Start Over
Effect of freezing sputum on Pseudomonas aeruginosa population heterogeneity.
- Source :
-
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society [J Cyst Fibros] 2017 May; Vol. 16 (3), pp. 353-357. Date of Electronic Publication: 2017 Jan 24. - Publication Year :
- 2017
-
Abstract
- Pseudomonas aeruginosa develops profound population heterogeneity in CF airways. How changes in these populations relate to clinical status is unknown. In order to facilitate this understanding, frequent sampling of this community is required. To determine if the collection and storage of sputum at home may pose a viable option, we collected sputum from ten patients. Sputum samples were partitioned in two, with half immediately processed on MacConkey agar and half assessed after freezing for one week in a home-freezer. From each sample, 88 isolates were assessed for antibiotic susceptibility and virulence factor production. Freezing resulted in a 10 <superscript>3</superscript> CFU/ml drop in P. aeruginosa. However, across 1760 isolates, no consistent difference in either antibiotic susceptibility nor virulence factors was observed suggesting freezing induced indiscriminate killing. Home collection and freezing of sputum will enable frequent and convenient assessment of P. aeruginosa population dynamics in CF.<br /> (Copyright © 2017 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Anti-Bacterial Agents classification
Anti-Bacterial Agents pharmacology
Freezing
Humans
Microbial Sensitivity Tests methods
Cryopreservation methods
Cystic Fibrosis complications
Cystic Fibrosis microbiology
Pseudomonas Infections diagnosis
Pseudomonas Infections drug therapy
Pseudomonas Infections etiology
Pseudomonas Infections microbiology
Pseudomonas aeruginosa isolation & purification
Specimen Handling methods
Sputum
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5010
- Volume :
- 16
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
- Publication Type :
- Academic Journal
- Accession number :
- 28126444
- Full Text :
- https://doi.org/10.1016/j.jcf.2017.01.004